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Phi80 JQ929581
(Plasmid #38170)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38170 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Phi80
  • Vector type
    Synthetic Biology ; Integration vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    pir2+
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    mKate2
  • Species
    Synthetic
  • Insert Size (bp)
    850

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Superfolder GFP
  • Species
    Synthetic
  • Insert Size (bp)
    812

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct has attB and attP sites (BP).

To integrate on your favorite E.coli strain, transform in strain containing the pAH123 plasmid (Haldimann et al., J of Bacteriology 2001). Contains a R6K origin of replication, will only replicate in pir2+ strains.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Phi80 JQ929581 was a gift from Drew Endy (Addgene plasmid # 38170 ; http://n2t.net/addgene:38170 ; RRID:Addgene_38170)
  • For your References section:

    Rewritable digital data storage in live cells via engineered control of recombination directionality. Bonnet J, Subsoontorn P, Endy D. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. 10.1073/pnas.1202344109 PubMed 22615351