β₂AR-mCFP
(Plasmid #38260)

• Depositing Lab: Robert Lefkowitz
• Publication: Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-88. Epub 2006 May 10.

Backbone

• Vector backbone: pcDNA3.1-mCFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: β₂AR
• Alt name: ADRB2
• Alt name: beta(2)AR
• Species: H. sapiens (human)
• Mutation: diglycine linker (replacing terminator codon)
• GenBank ID: NM_000024.5 NP_000015.1
• Entrez Gene: ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
• Promoter: CMV
• Tags / Fusion Proteins:
  • signal peptide sequence (MKTIIALSYIFCLVFA) (N terminal on insert)
  • Flag (N terminal on insert)
  • mCFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Lefkowitz Lab has confirmed this plasmid contains the human gene, and not the rat version described in the publication.