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phOct4-EGFP
(Plasmid #38776)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38776 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Oct-4 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3950
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
  • Promoter human OCT4
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer TT CCC ATG TCA AGT AAG TGG GGT GG
  • 3′ sequencing primer CGA GAA GGC AAA ATC TGA AGC CAG G
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The human OCT4 promoter (hOCT4pr, from 67539 to 71490 in human DNA sequence with accession number AP000509) was amplified by PCR with primers: hOCT4pr-F (5'−TTCCCATGTCAAGTAAGTGGGGTGG-3') and hOCT4pr-R (5'-CGAGAAGGCAAAATCTGAAGCCAGG-3') using human genomic DNA (Promega G3041) as a template. The fragment was cloned into a TOPO vector and the hOCT4 promoter was subsequently cloned into vector pEGFP1 by insertion into HindIII and AgeI restriction enzyme sites upstream of EGFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phOct4-EGFP was a gift from Wei Cui (Addgene plasmid # 38776)
  • For your References section:

    Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency. Gerrard L, Zhao D, Clark AJ, Cui W. Stem Cells. 2005;23(1):124-33. 10.1634/stemcells.2004-0102 PubMed 15625129