Addgene - pcDNA3.Flag.HERC2-HECT-WT.6xHis Plasmid Data

Plasmid 39227: pcDNA3.Flag.HERC2-HECT-WT.6xHis

Gene/insert name:
HERC2 HECT Domain
Alt name:
HERC2
Alt name:
HECT and RLD domain containing E3 ubiquitin protein ligase 2
Alt name:
p528
Insert size:
1000
Species:
H. sapiens (human)
GenBank ID:
NM_004667.5 Uniprot: O95714[4457-4794]
Entrez Gene:
HERC2 (D15F37S1, SHEP1, jdf2, p528)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Fusion protein or tag:
6xHis
Terminal:
C terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Promoter:
T7
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
Sp6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Aziz Sancar
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of nucleotide excision repair activity by transcriptional and post-transcriptional control of the XPA protein. Kang et al (Nucleic Acids Res. 2011 Apr;39(8):3176-87. Epub 2010 Dec 30. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 39227" in your Materials and Methods section.