Addgene - Flag-p53/pRK5 Plasmid Data

Plasmid 39237: Flag-p53/pRK5

Gene/insert name:
p53
Alt name:
p53
Insert size:
2591
Species:
H. sapiens (human)
Entrez Gene:
TP53 (BCC7, LFS1, P53, TRP53)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pRK5
(Search Vector Database)
Backbone manufacturer:
BD
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4754
Promoter:
CMV
Cloning method:
Unknown
5' sequencing primer:
N/A List of Sequencing Primers
3' sequencing primer:
N/A
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Xiaolu Yang
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: p53 regulates biosynthesis through direct inactivation of glucose-6-phosphate dehydrogenase. Jiang et al (Nat Cell Biol. 2011 Mar;13(3):310-6. Epub 2011 Feb 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 39237" in your Materials and Methods section.