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pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA
(Plasmid #39455)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 39455 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 5321
  • Total vector size (bp) 7523
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-hTet1CDmu
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2202
  • Mutation
    aa1418–2136 with mutations H1672 to Y, 1674D to A
  • Promoter EF1a
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer EF1A-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA was a gift from Hongjun Song (Addgene plasmid # 39455 ; http://n2t.net/addgene:39455 ; RRID:Addgene_39455)
  • For your References section:

    Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain. Guo JU, Su Y, Zhong C, Ming GL, Song H. Cell. 2011 Apr 29;145(3):423-34. Epub 2011 Apr 14. 10.1016/j.cell.2011.03.022 PubMed 21496894