Addgene - pLXSN-H-Ras_V12 Plasmid Data

Plasmid 39516: pLXSN-H-Ras_V12

Gene/insert name:
HRAS V12
Species:
H. sapiens (human)
Entrez Gene:
HRAS (C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1)
Mutation:
G12V
Vector backbone:
pLXSN
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Retroviral
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN_5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Julian Downward
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Role of phosphoinositide 3-OH kinase in cell transformation and control of the actin cytoskeleton by Ras. Rodriguez-Viciana et al (Cell. 1997 May 2;89(3):457-67. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 39516" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

39537: pLXSN-H-Ras_N17