pCyclinB1(L45A,R42A)-mCherry (1963)
(Plasmid #39852)

• Depositing Lab: Jonathon Pines
• Publication: Gavet et al J Cell Biol. 2010 Apr 19;189(2):247-59.

Backbone

• Vector backbone: pmCherry-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CyclinB1
• Species: H. sapiens (human)
• Mutation: L45A and R42A
• Entrez Gene: CCNB1 (a.k.a. CCNB)
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: mCherry Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry