pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)
(Plasmid #39858)

• Depositing Lab: Jonathon Pines
• Publication: Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-43. doi: 10.1038/ncb2347.

Backbone

• Vector backbone: pcDNA5-FRT/TO
• Backbone manufacturer: Invitrogen
• Modifications to backbone: Hygromycin resistance gene has been replaced with neomycin resistance gene.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: C11orf51
• Species: H. sapiens (human)
• Entrez Gene: ANAPC15 (a.k.a. APC15, C11orf51, HSPC020)
• Tag / Fusion Protein:
  • IRES2-mRuby (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI/PspOMI (destroyed during cloning)
• 5′ sequencing primer: LNCX primer
• 3′ sequencing primer: BGH_rev_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Can be used to rescue siC11orf51-4 from Pines lab.

C11orf51 and mRuby are separated by IRES2 and therefore are not fused.