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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti6.3-V5/DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7732
- Total vector size (bp) 8527
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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Alt nameGreen Fluorescent Protein
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Insert Size (bp)798
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer V5 reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6.3/V5-DEST-GFP was a gift from Lynda Chin (Addgene plasmid # 40125 ; http://n2t.net/addgene:40125 ; RRID:Addgene_40125) -
For your References section:
Integrative functional genomics identifies RINT1 as a novel GBM oncogene. Quayle SN, Chheda MG, Shukla SA, Wiedemeyer R, Tamayo P, Dewan RW, Zhuang L, Huang-Hobbs E, Haidar S, Xiao Y, Ligon KL, Hahn WC, Chin L. Neuro Oncol. 2012 Nov;14(11):1325-31. doi: 10.1093/neuonc/nos246. Epub 2012 Oct 16. 10.1093/neuonc/nos246 PubMed 23074196
Map uploaded by the depositor.
