pAM1414
(Plasmid #40237)

• Depositing Lab: Susan Golden
• Publication: Andersson et al Methods Enzymol. 2000;305:527-42.

Backbone

• Vector backbone: pAM1303
• Total vector size (bp): 8100
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): HB101
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: promoterless luxAB reporter
• Species: Synechococcus elongatus PCC 7942
• Insert Size (bp): 2400
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NS1 vector

Lux Genes: luxAB

cloning sites NotI and BamHI

Blunted (by Klenow) SalI-PvuII luxAB fragment (from pLAV1) was cloned in the SmaI site of pAM1303. SalI site is approximately 135bp upstream of luxA ATG within the luxD reading frame. You can clone promoters upstream of luxAB in NotI or BamHI sites.