pOPH6
(Plasmid #40315)

• Depositing Lab: Shaun Lott
• Publication: Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

Backbone

• Vector backbone: pBluescript KS(-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 4845
• Modifications to backbone: N-terminal OmpA3 leader sequence (for periplasmic localisation). C-terminal hexa-Histidine tag.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Peptide:N-glycosidase F
• Alt name: PNGase F
• Alt name: png
• Species: Flavobacterium meningosepticum
• Insert Size (bp): 1054
• GenBank ID: AF165910
• Promoter: T7
• Tags / Fusion Proteins:
  • HexaHistidine (C terminal on backbone)
  • OmpA3 leader (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry