pMIEG3-JunDN
(Plasmid #40350)

• Depositing Lab: Alexander Dent
• Publication: Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Backbone

• Vector backbone: pMIEG3
• Backbone manufacturer: David A. Williams (PMID: 10961859)
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Jun dominant negative
• Alt name: JunDN
• Alt name: c-Jun
• Species: H. sapiens (human)
• Mutation: first 122 amino acids deleted
• Entrez Gene: JUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
• Tags / Fusion Proteins:
  • HA (C terminal on insert)
  • IRES eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN_5
• 3′ sequencing primer: IRES-R2 (gacggcaatatggtggaaa)

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Jun dominant negative vector was made by first preparing a version of c-Jun with the first 122 aa deleted. This cDNA was amplified by PCR from human c-Jun with the following primers:

sense, 5-AAAAAA-GAATTC-ATGACTAGCCAGAACACGCTGCCCAGCGTC-3;

antisense, 5-AAAAAA-GAATTC-TCAGCTGGCATAGTCAGGCACGTCATAAGGATAGCTAAATGTTTGCAAC-3.

The antisense primer adds a hemagglutinin tag to the C terminus of c-Jun. The PCR product was then cut with EcoRI and cloned into the retroviral vector pMIEG3.