pMIEG3-NFAT2
(Plasmid #40351)

• Depositing Lab: Alexander Dent
• Publication: Wang et al J Immunol. 2006 Jan 15;176(2):880-8.

Backbone

• Vector backbone: pMIEG3
• Backbone manufacturer: David A. Williams (PMID: 10961859)
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NFAT2
• Alt name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
• Species: M. musculus (mouse)
• GenBank ID: NG_029226.1
• Entrez Gene: NFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
• Tag / Fusion Protein:
  • IRES eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: pLXSN_5
• 3′ sequencing primer: IRES-R2 (gacggcaatatggtggaaa)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The NFAT2-expressing retroviruses were constructed by inserting blunt-ended NFAT2 cDNA from pSH160C into the retroviral vector pMIEG3 via the blunt-ended XhoI site.