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FUGW-H1-Scrambled control shRNA
(Plasmid #40625)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40625 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FUGW-H1
  • Backbone size w/o insert (bp) 10130
  • Total vector size (bp) 10170
  • Vector type
    Lentiviral, RNAi
  • Selectable markers
    Zeocin ; Zeo marker is outside the LTRs and will not be packaged into virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Scrambled control shRNA
  • gRNA/shRNA sequence
    TTCTCCGAACGTGTCACGT
  • Species
    M. musculus (mouse)
  • GenBank ID

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This Scrambled control shRNA plasmid was generated by inserting the hairpin oligonucleotides into the FUGW-H1
lentiviral construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUGW-H1-Scrambled control shRNA was a gift from Sally Temple (Addgene plasmid # 40625 ; http://n2t.net/addgene:40625 ; RRID:Addgene_40625)
  • For your References section:

    The Niche Factor Syndecan-1 Regulates the Maintenance and Proliferation of Neural Progenitor Cells during Mammalian Cortical Development. Wang Q, Yang L, Alexander C, Temple S. PLoS One. 2012;7(8):e42883. Epub 2012 Aug 24. 10.1371/journal.pone.0042883 PubMed 22936997