pcDNA3-eGFP-LIC
(Plasmid #40768)

• Purpose: (Empty Backbone)
• Depositing Lab: Dustin Maly
• Publication: Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi: 10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3.

Backbone

• Vector backbone: pcDNA3
• Backbone size (bp): 6000
• Modifications to backbone: Parent vector is addgene #13031. N-terminal eGFP with ligation independent cloning site compatible with primers made for pMCSGx vectors (Midwest Center for Structural Genomics)
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: BGH-Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Parent is Addgene #13031
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry