|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40788||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 7053
Modifications to backboneAddition of TALEN motif with +63 C Terminus
Vector typeMammalian Expression, TALEN
Selectable markersNeomycin (select with G418) ; XGAL/IPTG
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTranscription Activator Like Effector Nuclease
Insert Size (bp)1625
Mutation+63 C Terminus, Codon Optimized FokI Endonuclease
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (not destroyed)
- 3′ cloning site BsmBI (not destroyed)
- 5′ sequencing primer GTAACAGCGGTAGAGGCAGTG
- 3′ sequencing primer CGTCCACCAAGACATGCCAAC (Common Sequencing Primers)
MR15 is a designation vector for the Cermak et al Golden Gate system designed for expression in mammalian cells.
Please acknowledge the principal investigator, Dr. Gang Bao, and include this article in your citations if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 40788" in your Materials and Methods section.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TALEN_C63 was a gift from Gang Bao (Addgene plasmid # 40788)