This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #40788)


Item Catalog # Description Quantity Price (USD)
Plasmid 40788 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7053
  • Modifications to backbone
    Addition of TALEN motif with +63 C Terminus
  • Vector type
    Mammalian Expression, TALEN
  • Selectable markers
    Neomycin (select with G418) ; XGAL/IPTG

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
  • Copy number
    High Copy


  • Gene/Insert name
    Transcription Activator Like Effector Nuclease
  • Alt name
  • Species
    Xanthamonas oryzae
  • Insert Size (bp)
  • Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (not destroyed)
  • 3′ cloning site BsmBI (not destroyed)
  • 5′ sequencing primer GTAACAGCGGTAGAGGCAGTG
  • 3′ sequencing primer CGTCCACCAAGACATGCCAAC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We modified the MR15 backbone provided by the Porteus lab at the Stanford University School of Medicine.
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

MR15 is a designation vector for the Cermak et al Golden Gate system designed for expression in mammalian cells.

Please acknowledge the principal investigator, Dr. Gang Bao, and include this article in your citations if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 40788" in your Materials and Methods section.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TALEN_C63 was a gift from Gang Bao (Addgene plasmid # 40788 ; ; RRID:Addgene_40788)