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pEGFP-N1-RACK1
(Plasmid #41088)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41088 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4733
  • Total vector size (bp) 5669
  • Modifications to backbone
    PCR-amplified insert was blunt-end ligated into pPCR-Script AMP vector, removed by digestion with HindIII and KpnI, and ligated into pEGFP-N1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Receptor for activated C kinase
  • Alt name
    RACK1
  • Alt name
    GNB2L1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    954
  • GenBank ID
    NM_006098.4
  • Entrez Gene
    RACK1 (a.k.a. GNB2L1, Gnb2-rs1, H12.3, HLC-7, PIG21)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • 3′ sequencing primer EGFP_C_primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pcDNA3.1 RACK1 was recieved from T.E. O'Toole at Scripps Research Institute [email protected] then cloned into N1-pEGFP
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-RACK1 was a gift from Anna Huttenlocher (Addgene plasmid # 41088 ; http://n2t.net/addgene:41088 ; RRID:Addgene_41088)
  • For your References section:

    RACK1 regulates integrin-mediated adhesion, protrusion, and chemotactic cell migration via its Src-binding site. Cox EA, Bennin D, Doan AT, O'Toole T, Huttenlocher A. Mol Biol Cell. 2003 Feb;14(2):658-69. 10.1091/mbc.e02-03-0142 PubMed 12589061