pCMVmyc-EPLIN beta 650-759
(Plasmid
#41090)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41090 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV-myc
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3800
- Total vector size (bp) 4200
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Modifications to backboneEco RI in MCS converted to Hind III
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEPLIN beta 650-759
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SpeciesH. sapiens (human)
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Insert Size (bp)327
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Mutationcontains amino acids 650-759
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Entrez GeneLIMA1 (a.k.a. EPLIN, LDLCQ8, SREBP3)
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMVmyc-EPLIN beta 650-759 was a gift from Elizabeth Luna (Addgene plasmid # 41090) -
For your References section:
Novel interactors and a role for supervillin in early cytokinesis. Smith TC, Fang Z, Luna EJ. Cytoskeleton (Hoboken). 2010 Jun;67(6):346-64. 10.1002/cm.20449 PubMed 20309963