pRcCMV myc-Plk1 T210D (Nigg LA183)
(Plasmid #41158)

• Depositing Lab: Erich Nigg
• Publication: Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Backbone

• Vector backbone: pRc/CMV
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5527
• Total vector size (bp): 7746
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Plk1
• Alt name: STPK13
• Alt name: polo-like kinase 1
• Alt name: serine/threonine-protein kinase 13
• Species: H. sapiens (human)
• Insert Size (bp): 2219
• Mutation: T210D
• GenBank ID: NM_005030.3 X73458.1
• Entrez Gene: PLK1 (a.k.a. PLK, STPK13)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry