pGFP-Cytochrome C
(Plasmid #41181)

• Depositing Lab: Douglas Green
• Publication: Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4725
• Total vector size (bp): 5026
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cytochrome C
• Alt name: cytog
• Species: H. sapiens (human)
• Insert Size (bp): 330
• Entrez Gene: CYCS (a.k.a. CYC, HCS, THC4)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: 5'-AGCACCCAGTCCGCCCTGAGC
• 3′ sequencing primer: 5'-GTAACCATTATAAGCTGCAATAAAC

Resource Information

• Supplemental Documents:
  • CytoG_Plasmid_Guide.pdf
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the original plasmid used in the Goldstein et al., NCB paper. However, the lab suggests that Addgene plasmids 41182 (transection) or 41183 and 41184 (retroviral) work better.