Addgene - pCAGGS-Flag-hsDicer Plasmid Data

Plasmid 41584: pCAGGS-Flag-hsDicer

Gene/insert name:
hsDicer
Alt name:
Dicer1
Insert size:
5796
Species:
H. sapiens (human)
GenBank ID:
NM_177438 NM_030621
Entrez Gene:
DICER1 (DCR1, Dicer, HERNA, MNG1, RMSE2)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Mutation:
None
Vector backbone:
pCAGGS
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4790
Promoter:
CMV
Cloning method:
Unknown
5' sequencing primer:
Custom List of Sequencing Primers
3' sequencing primer:
Custom
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Top10/P3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
None
Sequence: View sequences (2)
Map:
View map
Map:
View map
Principal Investigator:
Phil Sharp
Terms and Licenses:
UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs. Gurtan et al (RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 41584" in your Materials and Methods section.