pCAGGS-Flag-hsDicer
(Plasmid #41584)

• Depositing Lab: Phil Sharp
• Publication: Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30.
• Price: $65 (USD)

Backbone

• Vector backbone: pCAGGS
  (Search Vector Database)
• Backbone size w/o insert (bp): 4790
• Vector type: Mammalian Expression
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Copy number: High Copy

Sequence Information

• Depositor Sequences:
  Partial (1)
• Addgene Sequences:
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

• Gene/Insert name: hsDicer
• Alt name: Dicer1
• Species: H. sapiens (human)
• Insert Size (bp): 5796
• Mutation: None
• GenBank ID: NM_177438 NM_030621
• Entrez Gene: DICER1 (a.k.a. DCR1, Dicer, Dicer1e, HERNA, K12H4.8-LIKE, MNG1, RMSE2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Custom
• 3′ sequencing primer: Custom

Resource Information

• Terms and Licenses:
  • UBMTA