D10 pAcGP67a
(Plasmid
#41812)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAcGP67a
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Backbone manufacturerBD
- Backbone size w/o insert (bp) 9761
- Total vector size (bp) 10000
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameD10 Super-2
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Alt nameSuper-2
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Alt nameD10
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SpeciesH. sapiens (human)
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Insert Size (bp)429
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Entrez GeneIL2 (a.k.a. IL-2, TCGF, lymphokine)
- Promoter Polyhedron
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Tag
/ Fusion Protein
- 8x His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer PolyhedronFor
- 3′ sequencing primer PolyhedronRev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
D10 has the following mutations compared to wildtype IL-2: Q74H, L80F, R81F, L85V, I86V, I92F
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
D10 pAcGP67a was a gift from Chris Garcia (Addgene plasmid # 41812 ; http://n2t.net/addgene:41812 ; RRID:Addgene_41812) -
For your References section:
Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'. Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC. Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975. 10.1038/nature10975 PubMed 22446627