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pCTcon2
(Plasmid #41843)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41843 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCT
  • Backbone size w/o insert (bp) 6142
  • Total vector size (bp) 6456
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CD20
  • Insert Size (bp)
    183
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • c-myc (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid has been found to be prone to multimerization. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCTcon2 was a gift from Dane Wittrup (Addgene plasmid # 41843 ; http://n2t.net/addgene:41843 ; RRID:Addgene_41843)
  • For your References section:

    Isolating and engineering human antibodies using yeast surface display. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD. Nat Protoc. 2006;1(2):755-68. 10.1038/nprot.2006.94 PubMed 17406305