Venus-G protein Beta2 in pcDNA3.1
(Plasmid #42182)

• Depositing Lab: Narasimhan Gautam
• Publication: O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):E3568-77. doi: 10.1073/pnas.1205345109. Epub 2012 Dec 3.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6721
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein Beta 2
• Alt name: GnB2
• Species: H. sapiens (human)
• Insert Size (bp): 1293
• GenBank ID: NM_005273.3
• Entrez Gene: GNB2 (a.k.a. HG2C1, SSS4, SSS4; NEDHYDF)
• Promoter: CMV
• Tag / Fusion Protein:
  • Venus(156-239) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: BGH primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry