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pGL3-Basic-9xSeq2-Luc
(Plasmid #42214)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42214 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4859
  • Total vector size (bp) 5026
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    9xSeq2 Binding Sites
  • Insert Size (bp)
    159
  • Promoter 9 tandem copies of Seq2 + minimal human CMV promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CAGGTGCCAGAACATTTCTCT
  • 3′ sequencing primer GAGCTCTGCTTATATAGACCTCCC
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Binding site is AAACTGCAAAAG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Basic-9xSeq2-Luc was a gift from Charles Gersbach (Addgene plasmid # 42214)
  • For your References section:

    Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. Polstein LR, Gersbach CA. J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. Epub 2012 Sep 27. 10.1021/ja3065667 PubMed 22963237