pHYDuet93
(Plasmid #42303)

• Depositing Lab: Hideo Iwai
• Publication: Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi: 10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1.

Backbone

• Vector backbone: pSKDuet16
• Backbone size w/o insert (bp): 3992
• Total vector size (bp): 4392
• Modifications to backbone: introduced stop codon by inversion PCR to create NpuDnaEdC6-stop-
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NpuDnaEdC6
• Alt name: N-intein of NpuDnaEC6 split intein
• Alt name: split intein
• Species: Nostoc punctiforme
• Insert Size (bp): 400
• Promoter: T7
• Tag / Fusion Protein:
  • H6-GB1 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry