pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) Citations (178)

Originally described in: Multiplex Genome Engineering Using CRISPR/Cas Systems.
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F Science. 2013 Jan 3. PubMed Journal

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Articles Citing pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

Articles
Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. PubMed Associated Plasmids pAC1-pCR8-dCas9VP48 pAC84-pCR8-dCas9 pAC147-pCR8-dCas9VP64 pAC148-pCR8-dCas9VP96 pAC149-pCR8-dCas9VP160 More ...
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Wu X, Scott DA, Kriz AJ, Chiu AC, Hsu PD, Dadon DB, Cheng AW, Trevino AE, Konermann S, Chen S, Jaenisch R, Zhang F, Sharp PA. Nat Biotechnol. 2014 Jul;32(7):670-6. doi: 10.1038/nbt.2889. Epub 2014 Apr 20. PubMed
An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells. Munoz MC, Yanez DA, Stark JM. Nucleic Acids Res. 2014 Jul;42(12):7720-33. doi: 10.1093/nar/gku421. Epub 2014 May 14. PubMed
Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks. Renouf B, Piganeau M, Ghezraoui H, Jasin M, Brunet E. Methods Enzymol. 2014;546:251-71. doi: 10.1016/B978-0-12-801185-0.00012-X. PubMed
Genome engineering using CRISPR-Cas9 system. Cong L, Zhang F. Methods Mol Biol. 2015;1239:197-217. doi: 10.1007/978-1-4939-1862-1_10. PubMed
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Frock RL, Hu J, Meyers RM, Ho YJ, Kii E, Alt FW. Nat Biotechnol. 2015 Feb;33(2):179-86. doi: 10.1038/nbt.3101. Epub 2014 Dec 15. PubMed
Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing. Parikh BA, Beckman DL, Patel SJ, White JM, Yokoyama WM. PLoS One. 2015 Jan 14;10(1):e0116484. doi: 10.1371/journal.pone.0116484. eCollection 2015. PubMed
Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus. Merkle FT, Neuhausser WM, Santos D, Valen E, Gagnon JA, Maas K, Sandoe J, Schier AF, Eggan K. Cell Rep. 2015 May 12;11(6):875-83. doi: 10.1016/j.celrep.2015.04.007. Epub 2015 Apr 30. PubMed
Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system. Li S, Xue H, Long B, Sun L, Truong T, Liu Y. J Vis Exp. 2015 May 28;(99):e52539. doi: 10.3791/52539. PubMed
Simultaneous live imaging of the transcription and nuclear position of specific genes. Ochiai H, Sugawara T, Yamamoto T. Nucleic Acids Res. 2015 Jun 19. pii: gkv624. PubMed Associated Plasmids pBSKΔB-CAG-MCP-tdiRFP670-IRES-Neo pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo pBSKΔB-TRE-dCas9-EGFP pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP pTV-Oct4-TK-HMS
CRISPR-mediated genotypic and phenotypic correction of a chronic granulomatous disease mutation in human iPS cells. Flynn R, Grundmann A, Renz P, Hanseler W, James WS, Cowley SA, Moore MD. Exp Hematol. 2015 Oct;43(10):838-848.e3. doi: 10.1016/j.exphem.2015.06.002. Epub 2015 Jun 19. PubMed
CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells. Haraguchi M, Sato M, Ozawa M. PLoS One. 2015 Jul 10;10(7):e0132260. doi: 10.1371/journal.pone.0132260. eCollection 2015. PubMed
Nuclear domain 'knock-in' screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing. Pinder J, Salsman J, Dellaire G. Nucleic Acids Res. 2015 Oct 30;43(19):9379-92. doi: 10.1093/nar/gkv993. Epub 2015 Oct 1. PubMed Associated Plasmids pX330-LMNA-gRNA1 pCR2.1 Clover-LMNA Donor
Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1. Lai YC, Kondapalli C, Lehneck R, Procter JB, Dill BD, Woodroof HI, Gourlay R, Peggie M, Macartney TJ, Corti O, Corvol JC, Campbell DG, Itzen A, Trost M, Muqit MM. EMBO J. 2015 Nov 12;34(22):2840-61. doi: 10.15252/embj.201591593. Epub 2015 Oct 15. PubMed
Congenital Myasthenic Syndrome Type 19 Is Caused by Mutations in COL13A1, Encoding the Atypical Non-fibrillar Collagen Type XIII alpha1 Chain. Logan CV, Cossins J, Rodriguez Cruz PM, Parry DA, Maxwell S, Martinez-Martinez P, Riepsaame J, Abdelhamed ZA, Lake AV, Moran M, Robb S, Chow G, Sewry C, Hopkins PM, Sheridan E, Jayawant S, Palace J, Johnson CA, Beeson D. Am J Hum Genet. 2015 Dec 3;97(6):878-85. doi: 10.1016/j.ajhg.2015.10.017. Epub 2015 Nov 25. PubMed
Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. Wu J, Hunt SD, Xue H, Liu Y, Darabi R. Sci Rep. 2016 Jan 5;6:18759. doi: 10.1038/srep18759. PubMed
Generation and validation of PAX7 reporter lines from human iPS cells using CRISPR/Cas9 technology. Wu J, Hunt SD, Xue H, Liu Y, Darabi R. Stem Cell Res. 2016 Jan 13;16(2):220-228. doi: 10.1016/j.scr.2016.01.003. PubMed
Correction of the auditory phenotype in C57BL/6N mice via CRISPR/Cas9-mediated homology directed repair. Mianne J, Chessum L, Kumar S, Aguilar C, Codner G, Hutchison M, Parker A, Mallon AM, Wells S, Simon MM, Teboul L, Brown SD, Bowl MR. Genome Med. 2016 Feb 15;8(1):16. doi: 10.1186/s13073-016-0273-4. PubMed
Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases. Yang D, Scavuzzo MA, Chmielowiec J, Sharp R, Bajic A, Borowiak M. Sci Rep. 2016 Feb 18;6:21264. doi: 10.1038/srep21264. PubMed
Inactivation of prosurvival Bcl-2 proteins activates Bax/Bak through the outer mitochondrial membrane. O'Neill KL, Huang K, Zhang J, Chen Y, Luo X. Genes Dev. 2016 Apr 15;30(8):973-88. doi: 10.1101/gad.276725.115. Epub 2016 Apr 7. PubMed
Nipah Virus C Protein Recruits Tsg101 to Promote the Efficient Release of Virus in an ESCRT-Dependent Pathway. Park A, Yun T, Vigant F, Pernet O, Won ST, Dawes BE, Bartkowski W, Freiberg AN, Lee B. PLoS Pathog. 2016 May 20;12(5):e1005659. doi: 10.1371/journal.ppat.1005659. eCollection 2016 May. PubMed
Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Zhang J, Huang K, O'Neill KL, Pang X, Luo X. Cell Death Dis. 2016 Jun 16;7:e2266. doi: 10.1038/cddis.2016.167. PubMed
Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy. Heintze J, Costa JR, Weber M, Ketteler R. Cell Signal. 2016 Jun 18;28(9):1380-1388. doi: 10.1016/j.cellsig.2016.06.015. PubMed
Synthetic viability by BRCA2 and PARP1/ARTD1 deficiencies. Ding X, Ray Chaudhuri A, Callen E, Pang Y, Biswas K, Klarmann KD, Martin BK, Burkett S, Cleveland L, Stauffer S, Sullivan T, Dewan A, Marks H, Tubbs AT, Wong N, Buehler E, Akagi K, Martin SE, Keller JR, Nussenzweig A, Sharan SK. Nat Commun. 2016 Aug 8;7:12425. doi: 10.1038/ncomms12425. PubMed
Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours. Golias T, Papandreou I, Sun R, Kumar B, Brown NV, Swanson BJ, Pai R, Jaitin D, Le QT, Teknos TN, Denko NC. Sci Rep. 2016 Aug 8;6:31146. doi: 10.1038/srep31146. PubMed
Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Santos DP, Kiskinis E, Eggan K, Merkle FT. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15. PubMed
Enhancing a Wnt-Telomere Feedback Loop Restores Intestinal Stem Cell Function in a Human Organotypic Model of Dyskeratosis Congenita. Woo DH, Chen Q, Yang TL, Glineburg MR, Hoge C, Leu NA, Johnson FB, Lengner CJ. Cell Stem Cell. 2016 Sep 1;19(3):397-405. doi: 10.1016/j.stem.2016.05.024. Epub 2016 Aug 18. PubMed
The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells. Roncagalli R, Cucchetti M, Jarmuzynski N, Gregoire C, Bergot E, Audebert S, Baudelet E, Menoita MG, Joachim A, Durand S, Suchanek M, Fiore F, Zhang L, Liang Y, Camoin L, Malissen M, Malissen B. J Exp Med. 2016 Oct 17;213(11):2437-2457. Epub 2016 Sep 19. PubMed
An affinity-directed protein missile system for targeted proteolysis. Fulcher LJ, Macartney T, Bozatzi P, Hornberger A, Rojas-Fernandez A, Sapkota GP. Open Biol. 2016 Oct;6(10). pii: 160255. PubMed
Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression. Boehm E, Zornoza M, Jourdain AA, Delmiro Magdalena A, Garcia-Consuegra I, Torres Merino R, Orduna A, Martin MA, Martinou JC, De la Fuente MA, Simarro M. J Biol Chem. 2016 Dec 9;291(50):25877-25887. Epub 2016 Oct 27. PubMed
Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection. Perelman SS, Abrams ME, Eitson JL, Chen D, Jimenez A, Mettlen M, Schoggins JW, Alto NM. PLoS Pathog. 2016 Dec 21;12(12):e1006102. doi: 10.1371/journal.ppat.1006102. eCollection 2016 Dec. PubMed
CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Van Nostrand EL, Gelboin-Burkhart C, Wang R, Pratt GA, Blue SM, Yeo GW. Methods. 2016 Dec 18. pii: S1046-2023(16)30481-9. doi: 10.1016/j.ymeth.2016.12.007. PubMed
The Convenience of Single Homology Arm Donor DNA and CRISPR/Cas9-Nickase for Targeted Insertion of Long DNA Fragment. Basiri M, Behmanesh M, Tahamtani Y, Khalooghi K, Moradmand A, Baharvand H. Cell J. 2017 Winter;18(4):532-539. Epub 2016 Sep 26. PubMed
Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells. Sontag S, Forster M, Qin J, Wanek P, Mitzka S, Schuler HM, Koschmieder S, Rose-John S, Sere K, Zenke M. Stem Cells. 2017 Jan 16. doi: 10.1002/stem.2565. PubMed Associated Plasmids pX335A_hCas9(D10A)_IRF8gRNA1 pX335A_hCas9(D10A)_IRF8gRNA2 pX335A_hCas9(D10A)_IRF8gRNA3 pX335A_hCas9(D10A)_IRF8gRNA4
Genome Editing Reveals Glioblastoma Addiction to MicroRNA-10b. El Fatimy R, Subramanian S, Uhlmann EJ, Krichevsky AM. Mol Ther. 2017 Feb 1;25(2):368-378. doi: 10.1016/j.ymthe.2016.11.004. PubMed
Crosstalk between CLCb/Dyn1-Mediated Adaptive Clathrin-Mediated Endocytosis and Epidermal Growth Factor Receptor Signaling Increases Metastasis. Chen PH, Bendris N, Hsiao YJ, Reis CR, Mettlen M, Chen HY, Yu SL, Schmid SL. Dev Cell. 2017 Feb 6;40(3):278-288.e5. doi: 10.1016/j.devcel.2017.01.007. PubMed
Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9. Lv JN, Zhou GH, Chen X, Chen H, Wu KC, Xiang L, Lei XL, Zhang X, Wu RH, Jin ZB. Sci Rep. 2017 Feb 20;7:43062. doi: 10.1038/srep43062. PubMed
A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB. J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 10.1074/jbc.M117.777722. Epub 2017 Feb 22. PubMed Associated Plasmids BII-CPspaH2B BII-C3H BII-C3H2B BII-C3HTK BII-B3HTK More ...
CRISPR-Cas9 system-driven site-specific selection pressure on Herpes simplex virus genomes. Li Z, Bi Y, Xiao H, Sun L, Ren Y, Li Y, Chen C, Cun W. Virus Res. 2017 Mar 6. pii: S0168-1702(16)30694-3. doi: 10.1016/j.virusres.2017.03.010. PubMed
Regulation of membrane ruffling by polarized STIM1 and ORAI1 in cortactin-rich domains. Lopez-Guerrero AM, Tomas-Martin P, Pascual-Caro C, Macartney T, Rojas-Fernandez A, Ball G, Alessi DR, Pozo-Guisado E, Martin-Romero FJ. Sci Rep. 2017 Mar 24;7(1):383. doi: 10.1038/s41598-017-00331-4. PubMed
Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, Dekker J, Mirny LA, Bruneau BG. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. PubMed Associated Plasmids pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct pX335-EN475 pX335-EN477 pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26 pX330-EN479 More ...
LC3-association with the parasitophorous vacuole membrane of Plasmodium berghei liver stages follows a noncanonical autophagy pathway. Wacker R, Eickel N, Schmuckli-Maurer J, Annoura T, Niklaus L, Khan SM, Guan JL, Heussler VT. Cell Microbiol. 2017 Jun 1. doi: 10.1111/cmi.12754. PubMed
Statins do not inhibit the FGFR signaling in chondrocytes. Fafilek B, Hampl M, Ricankova N, Vesela I, Balek L, Kunova Bosakova M, Gudernova I, Varecha M, Buchtova M, Krejci P. Osteoarthritis Cartilage. 2017 Jun 3. pii: S1063-4584(17)31024-5. doi: 10.1016/j.joca.2017.05.014. PubMed
Infection exposure promotes ETV6-RUNX1 precursor B cell leukemia via impaired H3K4 demethylases. Rodriguez-Hernandez G, Hauer J, Martin-Lorenzo A, Schafer D, Bartenhagen C, Garcia-Ramirez I, Auer F, Gonzalez-Herrero I, Ruiz-Roca L, Gombert M, Okpanyi V, Fischer U, Chen C, Dugas M, Bhatia S, Linka RM, Garcia-Suquia M, Rascon-Trincado MV, Garcia-Sanchez A, Blanco O, Garcia-Cenador MB, Garcia-Criado FJ, Cobaleda C, Alonso-Lopez D, De Las Rivas J, Muschen M, Vicente-Duenas C, Sanchez-Garcia I, Borkhardt A. Cancer Res. 2017 Jun 19. pii: canres.0701.2017. doi: 10.1158/0008-5472.CAN-17-0701. PubMed
Generation and cardiac subtype-specific differentiation of PITX2-deficient human iPS cell lines for exploring familial atrial fibrillation. Marczenke M, Fell J, Piccini I, Ropke A, Seebohm G, Greber B. Stem Cell Res. 2017 May;21:26-28. doi: 10.1016/j.scr.2017.03.015. Epub 2017 Mar 23. PubMed
A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid. Talas A, Kulcsar PI, Weinhardt N, Borsy A, Toth E, Szebenyi K, Krausz SL, Huszar K, Vida I, Sturm A, Gordos B, Hoffmann OI, Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E. DNA Res. 2017 Jun 30. doi: 10.1093/dnares/dsx029. PubMed Associated Plasmids pSc1 pSc2 pSc1-puro pSc1-DD
Cardiac Subtype-Specific Modeling of Kv1.5 Ion Channel Deficiency Using Human Pluripotent Stem Cells. Marczenke M, Piccini I, Mengarelli I, Fell J, Ropke A, Seebohm G, Verkerk AO, Greber B. Front Physiol. 2017 Jul 6;8:469. doi: 10.3389/fphys.2017.00469. eCollection 2017. PubMed
Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo. Hanssen LLP, Kassouf MT, Oudelaar AM, Biggs D, Preece C, Downes DJ, Gosden M, Sharpe JA, Sloane-Stanley JA, Hughes JR, Davies B, Higgs DR. Nat Cell Biol. 2017 Aug;19(8):952-961. doi: 10.1038/ncb3573. Epub 2017 Jul 24. PubMed
Differential regulation of pro-inflammatory cytokine signalling by protein tyrosine phosphatases in pancreatic beta-cells. Stanley WJ, Trivedi PM, Sutherland AP, Thomas HE, Gurzov EN. J Mol Endocrinol. 2017 Nov;59(4):325-337. doi: 10.1530/JME-17-0089. Epub 2017 Aug 21. PubMed
Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane. Schmuckli-Maurer J, Reber V, Wacker R, Bindschedler A, Zakher A, Heussler VT. PLoS One. 2017 Aug 25;12(8):e0183797. doi: 10.1371/journal.pone.0183797. eCollection 2017. PubMed
Store-operated calcium entry is dispensable for the activation of ERK1/2 pathway in prostate cancer cells. Lopez-Guerrero AM, Pascual-Caro C, Martin-Romero FJ, Pozo-Guisado E. Cell Signal. 2017 Dec;40:44-52. doi: 10.1016/j.cellsig.2017.08.010. Epub 2017 Aug 31. PubMed
Loss of PRP4K drives anoikis resistance in part by dysregulation of epidermal growth factor receptor endosomal trafficking. Corkery DP, Clarke LE, Gebremeskel S, Salsman J, Pinder J, Le Page C, Meunier L, Xu Z, Mes-Masson AM, Berman JN, Johnston B, Dellaire G. Oncogene. 2018 Jan 11;37(2):174-184. doi: 10.1038/onc.2017.318. Epub 2017 Sep 11. PubMed
The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence. Mages CF, Wintsche A, Bernhart SH, Muller GA. Elife. 2017 Sep 18;6. doi: 10.7554/eLife.26876. PubMed
Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice. Miyauchi H, Ohta H, Nagaoka S, Nakaki F, Sasaki K, Hayashi K, Yabuta Y, Nakamura T, Yamamoto T, Saitou M. EMBO J. 2017 Nov 2;36(21):3100-3119. doi: 10.15252/embj.201796875. Epub 2017 Sep 19. PubMed
Adrenergic Stress Protection of Human iPS Cell-Derived Cardiomyocytes by Fast Kv7.1 Recycling. Piccini I, Fehrmann E, Frank S, Muller FU, Greber B, Seebohm G. Front Physiol. 2017 Sep 14;8:705. doi: 10.3389/fphys.2017.00705. eCollection 2017. PubMed
Evolutionarily Distinctive Transcriptional and Signaling Programs Drive Human Germ Cell Lineage Specification from Pluripotent Stem Cells. Kojima Y, Sasaki K, Yokobayashi S, Sakai Y, Nakamura T, Yabuta Y, Nakaki F, Nagaoka S, Woltjen K, Hotta A, Yamamoto T, Saitou M. Cell Stem Cell. 2017 Oct 5;21(4):517-532.e5. doi: 10.1016/j.stem.2017.09.005. PubMed
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. PubMed Associated Plasmids pmCherry_gRNA pX330-Flag-SpCas9-HF1 (without sgRNA) HeFm1SpCas9 HeFm2SpCas9 pX330-HA-dSpCas9 More ...
Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells. Schimmel J, Kool H, van Schendel R, Tijsterman M. EMBO J. 2017 Dec 15;36(24):3634-3649. doi: 10.15252/embj.201796948. Epub 2017 Oct 27. PubMed
Mechanism of activation of SGK3 by growth factors via the Class 1 and Class 3 PI3Ks. Malik N, Macartney T, Hornberger A, Anderson KE, Tovell H, Prescott AR, Alessi DR. Biochem J. 2018 Jan 2;475(1):117-135. doi: 10.1042/BCJ20170650. PubMed
Deletion of GIRK2 subunit containing GIRK channels of neurons expressing dopamine transporter decrease immobility time on forced swimming in mice. Honda I, Araki K, Honda S, Soeda F, Shin MC, Misumi S, Yamamura KI, Takahama K. Neurosci Lett. 2018 Feb 5;665:140-146. doi: 10.1016/j.neulet.2017.11.028. Epub 2017 Nov 24. PubMed
CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology. Wu WH, Tsai YT, Justus S, Cho GY, Sengillo JD, Xu Y, Cabral T, Lin CS, Bassuk AG, Mahajan VB, Tsang SH. Methods Mol Biol. 2018;1715:191-205. doi: 10.1007/978-1-4939-7522-8_13. PubMed
CRISPR/Cas9-mediated genome editing in naive human embryonic stem cells. Jacobs EZ, Warrier S, Volders PJ, D'haene E, Van Lombergen E, Vantomme L, Van der Jeught M, Heindryckx B, Menten B, Vergult S. Sci Rep. 2017 Nov 30;7(1):16650. doi: 10.1038/s41598-017-16932-y. PubMed
IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. Taulet N, Vitre B, Anguille C, Douanier A, Rocancourt M, Taschner M, Lorentzen E, Echard A, Delaval B. Nat Commun. 2017 Dec 4;8(1):1928. doi: 10.1038/s41467-017-01479-3. PubMed
Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. Adikusuma F, Pfitzner C, Thomas PQ. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. PubMed Associated Plasmids pDG330 pDG335 pDG458 pDG459 pDG461 More ...
Reversible Conformational Conversion of alpha-Synuclein into Toxic Assemblies by Glucosylceramide. Zunke F, Moise AC, Belur NR, Gelyana E, Stojkovska I, Dzaferbegovic H, Toker NJ, Jeon S, Fredriksen K, Mazzulli JR. Neuron. 2017 Dec 20. pii: S0896-6273(17)31135-2. doi: 10.1016/j.neuron.2017.12.012. PubMed
Revised roles of ISL1 in a hES cell-based model of human heart chamber specification. Quaranta R, Fell J, Ruhle F, Rao J, Piccini I, Arauzo-Bravo MJ, Verkerk AO, Stoll M, Greber B. Elife. 2018 Jan 16;7. doi: 10.7554/eLife.31706. PubMed
Necessity of p53-binding to the CDH1 locus for its expression defines two epithelial cell types differing in their integrity. Oikawa T, Otsuka Y, Onodera Y, Horikawa M, Handa H, Hashimoto S, Suzuki Y, Sabe H. Sci Rep. 2018 Jan 25;8(1):1595. doi: 10.1038/s41598-018-20043-7. PubMed
Chromosomal instability during neurogenesis in Huntington's disease. Ruzo A, Croft GF, Metzger JJ, Galgoczi S, Gerber LJ, Pellegrini C, Wang H Jr, Fenner M, Tse S, Marks A, Nchako C, Brivanlou AH. Development. 2018 Jan 29;145(2). pii: 145/2/dev156844. doi: 10.1242/dev.156844. PubMed
Cardiogenic programming of human pluripotent stem cells by dose-controlled activation of EOMES. Pfeiffer MJ, Quaranta R, Piccini I, Fell J, Rao J, Ropke A, Seebohm G, Greber B. Nat Commun. 2018 Jan 30;9(1):440. doi: 10.1038/s41467-017-02812-6. PubMed
Insights into the ubiquitin-proteasome system of human embryonic stem cells. Saez I, Koyuncu S, Gutierrez-Garcia R, Dieterich C, Vilchez D. Sci Rep. 2018 Mar 6;8(1):4092. doi: 10.1038/s41598-018-22384-9. PubMed
Paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption. Gopalappa R, Suresh B, Ramakrishna S, Kim HH. Nucleic Acids Res. 2018 Jul 6;46(12):e71. doi: 10.1093/nar/gky222. PubMed
A Transcriptional Circuit Filters Oscillating Circadian Hormonal Inputs to Regulate Fat Cell Differentiation. Bahrami-Nejad Z, Zhao ML, Tholen S, Hunerdosse D, Tkach KE, van Schie S, Chung M, Teruel MN. Cell Metab. 2018 Apr 3;27(4):854-868.e8. doi: 10.1016/j.cmet.2018.03.012. PubMed
IKK1/2 protect human cells from TNF-mediated RIPK1-dependent apoptosis in an NF-kappaB-independent manner. Slotta C, Storm J, Pfisterer N, Henkel E, Kleinwachter S, Pieper M, Ruiz-Perera LM, Greiner JFW, Kaltschmidt B, Kaltschmidt C. Biochim Biophys Acta Mol Cell Res. 2018 Aug;1865(8):1025-1033. doi: 10.1016/j.bbamcr.2018.04.003. Epub 2018 Apr 7. PubMed
Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription. Butepage M, Preisinger C, von Kriegsheim A, Scheufen A, Lausberg E, Li J, Kappes F, Feederle R, Ernst S, Eckei L, Krieg S, Muller-Newen G, Rossetti G, Feijs KLH, Verheugd P, Luscher B. Sci Rep. 2018 Apr 30;8(1):6748. doi: 10.1038/s41598-018-25137-w. PubMed
The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human. Mir R, Tonelli F, Lis P, Macartney T, Polinski NK, Martinez TN, Chou MY, Howden AJM, Konig T, Hotzy C, Milenkovic I, Brucke T, Zimprich A, Sammler E, Alessi DR. Biochem J. 2018 Jun 6;475(11):1861-1883. doi: 10.1042/BCJ20180248. PubMed
Self-organization of a human organizer by combined Wnt and Nodal signalling. Martyn I, Kanno TY, Ruzo A, Siggia ED, Brivanlou AH. Nature. 2018 Jun;558(7708):132-135. doi: 10.1038/s41586-018-0150-y. Epub 2018 May 23. PubMed
Generation and validation of homozygous fluorescent knock-in cells using CRISPR-Cas9 genome editing. Koch B, Nijmeijer B, Kueblbeck M, Cai Y, Walther N, Ellenberg J. Nat Protoc. 2018 Jun;13(6):1465-1487. doi: 10.1038/nprot.2018.042. Epub 2018 May 24. PubMed
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Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. Malik N, Nirujogi RS, Peltier J, Macartney T, Wightman M, Prescott AR, Gourlay R, Trost M, Alessi DR, Karapetsas A. Biochem J. 2019 Oct 30;476(20):3081-3107. doi: 10.1042/BCJ20190608. PubMed
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RAC1-Dependent ORAI1 Translocation to the Leading Edge Supports Lamellipodia Formation and Directional Persistence. Lopez-Guerrero AM, Espinosa-Bermejo N, Sanchez-Lopez I, Macartney T, Pascual-Caro C, Orantos-Aguilera Y, Rodriguez-Ruiz L, Perez-Oliva AB, Mulero V, Pozo-Guisado E, Martin-Romero FJ. Sci Rep. 2020 Apr 20;10(1):6580. doi: 10.1038/s41598-020-63353-5. PubMed
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PRDM8 reveals aberrant DNA methylation in aging syndromes and is relevant for hematopoietic and neuronal differentiation. Cypris O, Eipel M, Franzen J, Rosseler C, Tharmapalan V, Kuo CC, Vieri M, Nikolic M, Kirschner M, Brummendorf TH, Zenke M, Lampert A, Beier F, Wagner W. Clin Epigenetics. 2020 Aug 20;12(1):125. doi: 10.1186/s13148-020-00914-5. PubMed
RACK7 recognizes H3.3G34R mutation to suppress expression of MHC class II complex components and their delivery pathway in pediatric glioblastoma. Jiao F, Li Z, He C, Xu W, Yang G, Liu T, Shen H, Cai J, Anastas JN, Mao Y, Yu Y, Lan F, Shi YG, Jones C, Xu Y, Baker SJ, Shi Y, Guo R. Sci Adv. 2020 Jul 17;6(29):eaba2113. doi: 10.1126/sciadv.aba2113. eCollection 2020 Jul. PubMed
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CRISPR/Cas9-Based Knockout of GNAQ Reveals Differences in Host Cell Signaling Necessary for Egress of Apicomplexan Parasites. Burda PC, Bisio H, Marq JB, Soldati-Favre D, Heussler VT. mSphere. 2020 Dec 23;5(6). pii: 5/6/e01001-20. doi: 10.1128/mSphere.01001-20. PubMed
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Nintedanib targets KIT D816V neoplastic cells derived from induced pluripotent stem cells of systemic mastocytosis. Toledo MAS, Gatz M, Sontag S, Gleixner KV, Eisenwort G, Feldberg K, Hamouda AEI, Kluge F, Guareschi R, Rossetti G, Sechi AS, Dufva OMJ, Mustjoki SM, Maurer A, Schuler HM, Goetzke R, Braunschweig T, Kaiser A, Panse J, Jawhar M, Reiter A, Hilberg F, Ettmayer P, Wagner W, Koschmieder S, Brummendorf TH, Valent P, Chatain N, Zenke M. Blood. 2021 Apr 15;137(15):2070-2084. doi: 10.1182/blood.2019004509. PubMed
GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program. Kojima Y, Yamashiro C, Murase Y, Yabuta Y, Okamoto I, Iwatani C, Tsuchiya H, Nakaya M, Tsukiyama T, Nakamura T, Yamamoto T, Saitou M. Life Sci Alliance. 2021 Feb 19;4(5). pii: 4/5/e202000974. doi: 10.26508/lsa.202000974. Print 2021 May. PubMed
MAPRE2 mutations result in altered human cranial neural crest migration, underlying craniofacial malformations in CSC-KT syndrome. Thues C, Valadas JS, Deaulmerie L, Geens A, Chouhan AK, Duran-Romana R, Schymkowitz J, Rousseau F, Bartusel M, Rehimi R, Rada-Iglesias A, Verstreken P, Van Esch H. Sci Rep. 2021 Mar 2;11(1):4976. doi: 10.1038/s41598-021-83771-3. PubMed
A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor. Lu H, Liu J, Feng T, Guo Z, Yin Y, Gao F, Cao G, Du X, Wu S. Genome Res. 2021 May;31(5):900-909. doi: 10.1101/gr.271312.120. Epub 2021 Apr 1. PubMed
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The miR-26 family regulates neural differentiation-associated microRNAs and mRNAs by directly targeting REST. Sauer M, Was N, Ziegenhals T, Wang X, Hafner M, Becker M, Fischer U. J Cell Sci. 2021 Jun 15;134(12). pii: 269130. doi: 10.1242/jcs.257535. Epub 2021 Jun 21. PubMed
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Small tandem DNA duplications result from CST-guided Pol alpha-primase action at DNA break termini. Schimmel J, Munoz-Subirana N, Kool H, van Schendel R, Tijsterman M. Nat Commun. 2021 Aug 10;12(1):4843. doi: 10.1038/s41467-021-25154-w. PubMed
New hPSC SOX9 and INS Reporter Cell Lines Facilitate the Observation and Optimization of Differentiation into Insulin-Producing Cells. Dettmer R, Niwolik I, Mehmeti I, Jorns A, Naujok O. Stem Cell Rev Rep. 2021 Dec;17(6):2193-2209. doi: 10.1007/s12015-021-10232-9. Epub 2021 Aug 19. PubMed
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Adenine base editing of the DUX4 polyadenylation signal for targeted genetic therapy in facioscapulohumeral muscular dystrophy. Sikrova D, Cadar VA, Ariyurek Y, Laros JFJ, Balog J, van der Maarel SM. Mol Ther Nucleic Acids. 2021 Jun 1;25:342-354. doi: 10.1016/j.omtn.2021.05.020. eCollection 2021 Sep 3. PubMed
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