pET28a-His-hPKM2
(Plasmid #42515)

• Depositing Lab: Dimitrios Anastasiou
• Publication: Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. doi: 10.1126/science.1211485. Epub 2011 Nov 3.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: To produce protein, transform into BL21(DE3)pLysS cells.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Pyruvate Kinase M2
• Alt name: PKM2
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Entrez Gene: PKM (a.k.a. CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7 promoter primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned by Ning Wu in Lew Cantley's Lab.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Christofk, H. R., Vander Heiden, M. G., Wu, N., Asara, J. M., & Cantley, L. C. (2008). Pyruvate kinase M2 is a phosphotyrosine-binding protein. Nature, 452(7184), 181–186. doi:10.1038/nature06667