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phpRL-BCL2-FL-pA
(Plasmid #42595)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42595 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRL-HL
  • Backbone manufacturer
    Stanley Lemon (PubMed ID 10611164)
  • Total vector size (bp) 9596
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3900
  • Promoter CMV

Cloning Information

Resource Information

Depositor Comments

phpRL-BCL2-FL(pA) includes a 143-bp stable RNA hairpin located upstream of renilla luciferase, BCL2 5'UTR and firefly luciferase. See plasmid map link above for more details.

This plasmid is one of several reporter constructs created to assess potential IRES activity of the BCL2 5'UTR. The hairpin in this construct inhibits translation of renilla luciferase and to a lesser extent translation of firefly luciferase.

Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phpRL-BCL2-FL-pA was a gift from Richard Lloyd (Addgene plasmid # 42595 ; http://n2t.net/addgene:42595 ; RRID:Addgene_42595)
  • For your References section:

    BCL-2 translation is mediated via internal ribosome entry during cell stress. Sherrill KW, Byrd MP, Van Eden ME, Lloyd RE. J Biol Chem. 2004 Jul 9;279(28):29066-74. Epub 2004 May 3. 10.1074/jbc.M402727200 PubMed 15123638