pGC480
(Plasmid #42600)

• Depositing Lab: Jane Hubbard
• Publication: Korta et al Development. 2012 Mar;139(5):859-70. doi: 10.1242/dev.074047. Epub 2012 Jan 25.

Backbone

• Vector backbone: L4440
• Backbone size w/o insert (bp): 2790
• Total vector size (bp): 3838
• Vector type: RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow on LB + Amp + Tet plates. For RNAi feeding of worms grow LB liquid cultures 16 hours with only Amp.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: let-363 cDNA
• gRNA/shRNA sequence: let-363
• Species: C. elegans (nematode)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.