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pcDNA3-APX(W41F)-NES
(Plasmid #42608)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42608 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Total vector size (bp) 6198
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    soybean APX W41F (dimeric) -NES
  • Species
    soybean
  • Insert Size (bp)
    810
  • Mutation
    W41F mutation
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVf
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is for expression of soybean APX W41F (dimeric) in mammalian cells under the CMV promoter. In this construct, APX has a C-terminal nuclear export signal (NES), with amino acid sequence LQLPPLERLTLD.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-APX(W41F)-NES was a gift from Alice Ting (Addgene plasmid # 42608 ; http://n2t.net/addgene:42608 ; RRID:Addgene_42608)
  • For your References section:

    Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging. Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Science. 2013 Jan 31. 10.1126/science.1230593 PubMed 23371551