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pPROEX Aqua
(Plasmid #42889)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42889 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pProEX HTa
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4751
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Aquamarine
  • Alt name
    ECFP-T65S-H148G
  • Species
    A. victoria
  • Mutation
    T65S and H148G
  • Tag / Fusion Protein
    • 6xHis and TEV cleavage (N terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPROEX Aqua was a gift from Fabienne Merola (Addgene plasmid # 42889 ; http://n2t.net/addgene:42889 ; RRID:Addgene_42889)
  • For your References section:

    Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging. Erard M, Fredj A, Pasquier H, Beltolngar DB, Bousmah Y, Derrien V, Vincent P, Merola F. Mol Biosyst. 2013 Feb 2;9(2):258-67. doi: 10.1039/c2mb25303h. Epub 2012 Nov 28. 10.1039/c2mb25303h PubMed 23192565