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MM39
(Bacterial strain #42894)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 42894 Bacteria in agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    genome

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MM39
  • Growth instructions
    Selection using 10-15 micrograms/mL (ug/mL)
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

selection using 10-15 micrograms/mL strep.

It is MalE deficient, which enables maltose complementation. For the assay, a particular construct is transformed into MM39 and grown on maltose minimal media to confirm proper membrane orientation and integration.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MM39 was a gift from Bryan Berger (Addgene plasmid # 42894)
  • For your References section:

    Identifying Key Juxtamembrane Interactions in Cell Membranes Using AraTM. Su PC, Berger BW. J Biol Chem. 2012 Jul 22. 10.1074/jbc.M112.396895 PubMed 22822084