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pPET-PKR/PPase
(Plasmid #42934)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42934 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pet11a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    protein kinase R
  • Alt name
    PKR
  • Alt name
    EIF2AK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1653
  • GenBank ID
    NM_002759.3
  • Entrez Gene
    EIF2AK2 (a.k.a. DYT33, EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promotor
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    phage lambda phosphatase
  • Species
    phage lambda
  • Insert Size (bp)
    663

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BlpI (not destroyed)
  • 5′ sequencing primer T7 promotor
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPET-PKR/PPase was a gift from James Cole (Addgene plasmid # 42934 ; http://n2t.net/addgene:42934 ; RRID:Addgene_42934)
  • For your References section:

    Mechanism of PKR activation: dimerization and kinase activation in the absence of double-stranded RNA. Lemaire PA, Lary J, Cole JL. J Mol Biol. 2005 Jan 7;345(1):81-90. 10.1016/j.jmb.2004.10.031 PubMed 15567412