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MLM3636 Citations (113)

Originally described in: Joung lab unpublished CRISPR plasmids
Joung JK Unpublished

Articles Citing MLM3636

Articles
Direct activation of human and mouse Oct4 genes using engineered TALE and Cas9 transcription factors. Hu J, Lei Y, Wong WK, Liu S, Lee KC, He X, You W, Zhou R, Guo JT, Chen X, Peng X, Sun H, Huang H, Zhao H, Feng B. Nucleic Acids Res. 2014 Apr;42(7):4375-90. doi: 10.1093/nar/gku109. Epub 2014 Feb 5. PubMed
Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System. Xue H, Wu J, Li S, Rao MS, Liu Y. Methods Mol Biol. 2014 Mar 11. PubMed
Chromosomal translocations in human cells are generated by canonical nonhomologous end-joining. Ghezraoui H, Piganeau M, Renouf B, Renaud JB, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, Brunet E. Mol Cell. 2014 Sep 18;55(6):829-42. doi: 10.1016/j.molcel.2014.08.002. Epub 2014 Sep 4. PubMed
A prominent and conserved role for YY1 in Xist transcriptional activation. Makhlouf M, Ouimette JF, Oldfield A, Navarro P, Neuillet D, Rougeulle C. Nat Commun. 2014 Sep 11;5:4878. doi: 10.1038/ncomms5878. PubMed
Mouse Genome Editing Using the CRISPR/Cas System. Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB. Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83. PubMed

Associated Plasmids

CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish. Auer TO, Duroure K, Concordet JP, Del Bene F. Nat Protoc. 2014 Dec;9(12):2823-40. doi: 10.1038/nprot.2014.187. Epub 2014 Nov 13. PubMed
Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs. Fu Y, Reyon D, Joung JK. Methods Enzymol. 2014;546:21-45. doi: 10.1016/B978-0-12-801185-0.00002-7. PubMed
Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks. Renouf B, Piganeau M, Ghezraoui H, Jasin M, Brunet E. Methods Enzymol. 2014;546:251-71. doi: 10.1016/B978-0-12-801185-0.00012-X. PubMed
CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on the Proteome. Mehrabian M, Brethour D, MacIsaac S, Kim JK, Gunawardana CG, Wang H, Schmitt-Ulms G. PLoS One. 2014 Dec 9;9(12):e114594. doi: 10.1371/journal.pone.0114594. eCollection 2014. PubMed

Associated Plasmids

Hypothetical gene C18orf42 encodes a novel protein kinase A-binding protein. Fukuda M, Aizawa Y. Genes Cells. 2015 Apr;20(4):267-80. doi: 10.1111/gtc.12217. Epub 2015 Feb 4. PubMed
Contributions of unique intracellular domains to switchlike biosensing by Toll-like receptor 4. Daringer NM, Schwarz KA, Leonard JN. J Biol Chem. 2015 Apr 3;290(14):8764-77. doi: 10.1074/jbc.M114.610063. Epub 2015 Feb 18. PubMed
Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis. Seruggia D, Fernandez A, Cantero M, Pelczar P, Montoliu L. Nucleic Acids Res. 2015 May 26;43(10):4855-67. doi: 10.1093/nar/gkv375. Epub 2015 Apr 20. PubMed
Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system. Li S, Xue H, Long B, Sun L, Truong T, Liu Y. J Vis Exp. 2015 May 28;(99):e52539. doi: 10.3791/52539. PubMed
Human Induced Pluripotent Stem Cell NEUROG2 Dual Knockin Reporter Lines Generated by the CRISPR/Cas9 System. Li S, Xue H, Wu J, Rao MS, Kim DH, Deng W, Liu Y. Stem Cells Dev. 2015 Dec 15;24(24):2925-42. doi: 10.1089/scd.2015.0131. Epub 2015 Nov 5. PubMed
Genome Editing in Human Cells Using CRISPR/Cas Nucleases. Wyvekens N, Tsai SQ, Joung JK. Curr Protoc Mol Biol. 2015 Oct 1;112:31.3.1-31.3.18. doi: 10.1002/0471142727.mb3103s112. PubMed
Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins. Menoret S, De Cian A, Tesson L, Remy S, Usal C, Boule JB, Boix C, Fontaniere S, Creneguy A, Nguyen TH, Brusselle L, Thinard R, Gauguier D, Concordet JP, Cherifi Y, Fraichard A, Giovannangeli C, Anegon I. Sci Rep. 2015 Oct 7;5:14410. doi: 10.1038/srep14410. PubMed
A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneurons. Xu H, Iyer N, Huettner JE, Sakiyama-Elbert SE. Stem Cell Res Ther. 2015 Nov 10;6:220. doi: 10.1186/s13287-015-0213-z. PubMed
Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome. Muller M, Lee CM, Gasiunas G, Davis TH, Cradick TJ, Siksnys V, Bao G, Cathomen T, Mussolino C. Mol Ther. 2015 Dec 14. doi: 10.1038/mt.2015.218. PubMed
CCR5 Disruption in Induced Pluripotent Stem Cells Using CRISPR/Cas9 Provides Selective Resistance of Immune Cells to CCR5-tropic HIV-1 Virus. Kang H, Minder P, Park MA, Mesquitta WT, Torbett BE, Slukvin II. Mol Ther Nucleic Acids. 2015 Dec 15;4:e268. doi: 10.1038/mtna.2015.42. PubMed
Generation of Highly Enriched V2a Interneurons from Mouse Embryonic Stem Cells. Iyer NR, Huettner JE, Butts JC, Brown CR, Sakiyama-Elbert SE. Exp Neurol. 2016 Jan 16. pii: S0014-4886(16)30009-7. doi: 10.1016/j.expneurol.2016.01.011. PubMed
Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation. McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards JR, Challen GA. Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067. PubMed

Associated Plasmids

Preparation and Analysis of Native Chromatin-Modifying Complexes. Doyon Y, Cote J. Methods Enzymol. 2016;573:303-18. doi: 10.1016/bs.mie.2016.01.017. Epub 2016 Mar 16. PubMed
Writing of H3K4Me3 overcomes epigenetic silencing in a sustained but context-dependent manner. Cano-Rodriguez D, Gjaltema RA, Jilderda LJ, Jellema P, Dokter-Fokkens J, Ruiters MH, Rots MG. Nat Commun. 2016 Aug 10;7:12284. doi: 10.1038/ncomms12284. PubMed
Exploiting the CRISPR/Cas9 system to study alternative splicing in vivo: application to titin. Charton K, Suel L, Henriques SF, Moussu JP, Bovolenta M, Taillepierre M, Becker C, Lipson K, Richard I. Hum Mol Genet. 2016 Aug 23. pii: ddw280. PubMed
Identification of Small-Molecule PHD2 Zinc Finger Inhibitors that Activate Hypoxia Inducible Factor. Arsenault PR, Song D, Bergkamp M, Ravaschiere AM, Navalsky BE, Lieberman PM, Lee FS. Chembiochem. 2016 Dec 14;17(24):2316-2323. doi: 10.1002/cbic.201600493. Epub 2016 Nov 11. PubMed
CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening. Koferle A, Worf K, Breunig C, Baumann V, Herrero J, Wiesbeck M, Hutter LH, Gotz M, Fuchs C, Beck S, Stricker SH. BMC Genomics. 2016 Nov 14;17(1):917. PubMed
Rewiring human cellular input-output using modular extracellular sensors. Schwarz KA, Daringer NM, Dolberg TB, Leonard JN. Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253. PubMed

Associated Plasmids

Targeted epigenetic editing of SPDEF reduces mucus production in lung epithelial cells. Song J, Cano Rodriguez D, Winkle M, Gjaltema RA, Goubert D, Jurkowski TP, Heijink IH, Rots MG, Hylkema MN. Am J Physiol Lung Cell Mol Physiol. 2016 Dec 23:ajplung.00059.2016. doi: 10.1152/ajplung.00059.2016. PubMed
Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus. Bothmer A, Phadke T, Barrera LA, Margulies CM, Lee CS, Buquicchio F, Moss S, Abdulkerim HS, Selleck W, Jayaram H, Myer VE, Cotta-Ramusino C. Nat Commun. 2017 Jan 9;8:13905. doi: 10.1038/ncomms13905. PubMed
A ZIP6-ZIP10 heteromer controls NCAM1 phosphorylation and integration into focal adhesion complexes during epithelial-to-mesenchymal transition. Brethour D, Mehrabian M, Williams D, Wang X, Ghodrati F, Ehsani S, Rubie EA, Woodgett JR, Sevalle J, Xi Z, Rogaeva E, Schmitt-Ulms G. Sci Rep. 2017 Jan 18;7:40313. doi: 10.1038/srep40313. PubMed
Precise and efficient scarless genome editing in stem cells using CORRECT. Kwart D, Paquet D, Teo S, Tessier-Lavigne M. Nat Protoc. 2017 Feb;12(2):329-354. doi: 10.1038/nprot.2016.171. Epub 2017 Jan 19. PubMed
Exploiting the CRISPR/Cas9 system to study alternative splicing in vivo: application to titin. Charton K, Suel L, Henriques SF, Moussu JP, Bovolenta M, Taillepierre M, Becker C, Lipson K, Richard I. Hum Mol Genet. 2016 Oct 15;25(20):4518-4532. doi: 10.1093/hmg/ddw280. PubMed
Mechanisms of precise genome editing using oligonucleotide donors. Kan Y, Ruis B, Takasugi T, Hendrickson EA. Genome Res. 2017 Jul;27(7):1099-1111. doi: 10.1101/gr.214775.116. Epub 2017 Mar 29. PubMed
Sensing Self and Foreign Circular RNAs by Intron Identity. Chen YG, Kim MV, Chen X, Batista PJ, Aoyama S, Wilusz JE, Iwasaki A, Chang HY. Mol Cell. 2017 Jul 20;67(2):228-238.e5. doi: 10.1016/j.molcel.2017.05.022. Epub 2017 Jun 15. PubMed
Reconstruction of enhancer-target networks in 935 samples of human primary cells, tissues and cell lines. Cao Q, Anyansi C, Hu X, Xu L, Xiong L, Tang W, Mok MTS, Cheng C, Fan X, Gerstein M, Cheng ASL, Yip KY. Nat Genet. 2017 Oct;49(10):1428-1436. doi: 10.1038/ng.3950. Epub 2017 Sep 4. PubMed
CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons. Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R, Ciccia A. Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 10.1016/j.molcel.2017.08.008. PubMed

Associated Plasmids

miR-155 induces ROS generation through downregulation of antioxidation-related genes in mesenchymal stem cells. Onodera Y, Teramura T, Takehara T, Obora K, Mori T, Fukuda K. Aging Cell. 2017 Dec;16(6):1369-1380. doi: 10.1111/acel.12680. Epub 2017 Oct 2. PubMed
Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells. Ade CM, Derbes RS, Wagstaff BJ, Linker SB, White TB, Deharo D, Belancio VP, Ivics Z, Roy-Engel AM. Gene. 2018 Feb 5;642:188-198. doi: 10.1016/j.gene.2017.11.033. Epub 2017 Nov 14. PubMed
Optimized guide RNA structure for genome editing via Cas9. Xu J, Lian W, Jia Y, Li L, Huang Z. Oncotarget. 2017 Oct 7;8(55):94166-94171. doi: 10.18632/oncotarget.21607. eCollection 2017 Nov 7. PubMed
A Survey of Validation Strategies for CRISPR-Cas9 Editing. Sentmanat MF, Peters ST, Florian CP, Connelly JP, Pruett-Miller SM. Sci Rep. 2018 Jan 17;8(1):888. doi: 10.1038/s41598-018-19441-8. PubMed
An "off-the-shelf" fratricide-resistant CAR-T for the treatment of T cell hematologic malignancies. Cooper ML, Choi J, Staser K, Ritchey JK, Devenport JM, Eckardt K, Rettig MP, Wang B, Eissenberg LG, Ghobadi A, Gehrs LN, Prior JL, Achilefu S, Miller CA, Fronick CC, O'Neal J, Gao F, Weinstock DM, Gutierrez A, Fulton RS, DiPersio JF. Leukemia. 2018 Sep;32(9):1970-1983. doi: 10.1038/s41375-018-0065-5. Epub 2018 Feb 20. PubMed
Establishment of Cell Lines Stably Expressing dCas9-Fusions to Address Kinetics of Epigenetic Editing. Goubert D, Koncz M, Kiss A, Rots MG. Methods Mol Biol. 2018;1767:395-415. doi: 10.1007/978-1-4939-7774-1_22. PubMed

Associated Plasmids

CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Charpentier M, Khedher AHY, Menoret S, Brion A, Lamribet K, Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A, Itier JM, Anegon I, Lopez B, Giovannangeli C, Concordet JP. Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7. PubMed

Associated Plasmids

A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. St Martin A, Salamango D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. PubMed

Associated Plasmids

Efficient CRISPR/Cas9-mediated editing of trinucleotide repeat expansion in myotonic dystrophy patient-derived iPS and myogenic cells. Dastidar S, Ardui S, Singh K, Majumdar D, Nair N, Fu Y, Reyon D, Samara E, Gerli MFM, Klein AF, De Schrijver W, Tipanee J, Seneca S, Tulalamba W, Wang H, Chai YC, In't Veld P, Furling D, Tedesco FS, Vermeesch JR, Joung JK, Chuah MK, VandenDriessche T. Nucleic Acids Res. 2018 Sep 19;46(16):8275-8298. doi: 10.1093/nar/gky548. PubMed
In vitro effects of FBXW7 mutation in serous endometrial cancer: Increased levels of potentially druggable proteins and sensitivity to SI-2 and dinaciclib. Urick ME, Bell DW. Mol Carcinog. 2018 Nov;57(11):1445-1457. doi: 10.1002/mc.22867. Epub 2018 Jul 12. PubMed
Optimized Plasmid Construction Strategy for Cas9. Xu J, Li W, Hossen MM, Jia Y, Li L, Huang Z. Cell Physiol Biochem. 2018;48(1):131-137. doi: 10.1159/000491669. Epub 2018 Jul 12. PubMed
Ex Vivo COL7A1 Correction for Recessive Dystrophic Epidermolysis Bullosa Using CRISPR/Cas9 and Homology-Directed Repair. Izmiryan A, Ganier C, Bovolenta M, Schmitt A, Mavilio F, Hovnanian A. Mol Ther Nucleic Acids. 2018 Sep 7;12:554-567. doi: 10.1016/j.omtn.2018.06.008. Epub 2018 Jun 26. PubMed
Tissue-specific expression and post-transcriptional regulation of the ATPase inhibitory factor 1 (IF1) in human and mouse tissues. Esparza-Molto PB, Nuevo-Tapioles C, Chamorro M, Najera L, Torresano L, Santacatterina F, Cuezva JM. FASEB J. 2019 Feb;33(2):1836-1851. doi: 10.1096/fj.201800756R. Epub 2018 Sep 11. PubMed
Regulatory mechanisms of incomplete huntingtin mRNA splicing. Neueder A, Dumas AA, Benjamin AC, Bates GP. Nat Commun. 2018 Sep 27;9(1):3955. doi: 10.1038/s41467-018-06281-3. PubMed

Associated Plasmids

Angiomotins stimulate LATS kinase autophosphorylation and act as scaffolds that promote Hippo signaling. Mana-Capelli S, McCollum D. J Biol Chem. 2018 Nov 23;293(47):18230-18241. doi: 10.1074/jbc.RA118.004187. Epub 2018 Sep 28. PubMed
Inflammation-associated miR-155 activates differentiation of muscular satellite cells. Onodera Y, Teramura T, Takehara T, Itokazu M, Mori T, Fukuda K. PLoS One. 2018 Oct 1;13(10):e0204860. doi: 10.1371/journal.pone.0204860. eCollection 2018. PubMed
A bispecific immunotweezer prevents soluble PrP oligomers and abolishes prion toxicity. Bardelli M, Frontzek K, Simonelli L, Hornemann S, Pedotti M, Mazzola F, Carta M, Eckhardt V, D'Antuono R, Virgilio T, Gonzalez SF, Aguzzi A, Varani L. PLoS Pathog. 2018 Oct 1;14(10):e1007335. doi: 10.1371/journal.ppat.1007335. eCollection 2018 Oct. PubMed
CRISPR deletion of MIEN1 in breast cancer cells. Van Treuren T, Vishwanatha JK. PLoS One. 2018 Oct 4;13(10):e0204976. doi: 10.1371/journal.pone.0204976. eCollection 2018. PubMed
Optimization of CRISPR/Cas9 Delivery to Human Hematopoietic Stem and Progenitor Cells for Therapeutic Genomic Rearrangements. Lattanzi A, Meneghini V, Pavani G, Amor F, Ramadier S, Felix T, Antoniani C, Masson C, Alibeu O, Lee C, Porteus MH, Bao G, Amendola M, Mavilio F, Miccio A. Mol Ther. 2019 Jan 2;27(1):137-150. doi: 10.1016/j.ymthe.2018.10.008. Epub 2018 Oct 17. PubMed
Aberrant enhancer hypomethylation contributes to hepatic carcinogenesis through global transcriptional reprogramming. Xiong L, Wu F, Wu Q, Xu L, Cheung OK, Kang W, Mok MT, Szeto LLM, Lun CY, Lung RW, Zhang J, Yu KH, Lee SD, Huang G, Wang CM, Liu J, Yu Z, Yu DY, Chou JL, Huang WH, Feng B, Cheung YS, Lai PB, Tan P, Wong N, Chan MW, Huang TH, Yip KY, Cheng AS, To KF. Nat Commun. 2019 Jan 18;10(1):335. doi: 10.1038/s41467-018-08245-z. PubMed
A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris RS. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. PubMed

Associated Plasmids

Edition of TFAM gene by CRISPR/Cas9 technology in bovine model. de Oliveira VC, Moreira GSA, Bressan FF, Gomes Mariano Junior C, Roballo KCS, Charpentier M, Concordet JP, Meirelles FV, Ambrosio CE. PLoS One. 2019 Mar 7;14(3):e0213376. doi: 10.1371/journal.pone.0213376. eCollection 2019. PubMed
Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA. Konig S, Yang Z, Wandall HH, Mussolino C, Bennett EP. Methods Mol Biol. 2019;1961:45-66. doi: 10.1007/978-1-4939-9170-9_4. PubMed
ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Wang B, Maxwell BA, Joo JH, Gwon Y, Messing J, Mishra A, Shaw TI, Ward AL, Quan H, Sakurada SM, Pruett-Miller SM, Bertorini T, Vogel P, Kim HJ, Peng J, Taylor JP, Kundu M. Mol Cell. 2019 May 16;74(4):742-757.e8. doi: 10.1016/j.molcel.2019.03.027. Epub 2019 Apr 9. PubMed
Involvement of ADAM12 in Chondrocyte Differentiation by Regulation of TGF-beta1-Induced IGF-1 and RUNX-2 Expressions. Horita M, Nishida K, Hasei J, Furumatsu T, Sakurai M, Onodera Y, Fukuda K, Salter DM, Ozaki T. Calcif Tissue Int. 2019 Jul;105(1):97-106. doi: 10.1007/s00223-019-00549-6. Epub 2019 Apr 16. PubMed
Tailoring the CRISPR system to transactivate coagulation gene promoters in normal and mutated contexts. Pignani S, Zappaterra F, Barbon E, Follenzi A, Bovolenta M, Bernardi F, Branchini A, Pinotti M. Biochim Biophys Acta Gene Regul Mech. 2019 Jun;1862(6):619-624. doi: 10.1016/j.bbagrm.2019.04.002. Epub 2019 Apr 18. PubMed
Targeted deletion of BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta thalassemia disease. Khosravi MA, Abbasalipour M, Concordet JP, Berg JV, Zeinali S, Arashkia A, Azadmanesh K, Buch T, Karimipoor M. Eur J Pharmacol. 2019 Jul 5;854:398-405. doi: 10.1016/j.ejphar.2019.04.042. Epub 2019 Apr 27. PubMed
Repression of TERRA Expression by Subtelomeric DNA Methylation Is Dependent on NRF1 Binding. Le Berre G, Hossard V, Riou JF, Guieysse-Peugeot AL. Int J Mol Sci. 2019 Jun 7;20(11). pii: ijms20112791. doi: 10.3390/ijms20112791. PubMed
Involvement of G-quadruplex regions in mammalian replication origin activity. Prorok P, Artufel M, Aze A, Coulombe P, Peiffer I, Lacroix L, Guedin A, Mergny JL, Damaschke J, Schepers A, Ballester B, Mechali M. Nat Commun. 2019 Jul 22;10(1):3274. doi: 10.1038/s41467-019-11104-0. PubMed
A Large Panel of Isogenic APP and PSEN1 Mutant Human iPSC Neurons Reveals Shared Endosomal Abnormalities Mediated by APP beta-CTFs, Not Abeta. Kwart D, Gregg A, Scheckel C, Murphy EA, Paquet D, Duffield M, Fak J, Olsen O, Darnell RB, Tessier-Lavigne M. Neuron. 2019 Oct 23;104(2):256-270.e5. doi: 10.1016/j.neuron.2019.07.010. Epub 2019 Aug 12. PubMed
PPM1D mutations silence NAPRT gene expression and confer NAMPT inhibitor sensitivity in glioma. Fons NR, Sundaram RK, Breuer GA, Peng S, McLean RL, Kalathil AN, Schmidt MS, Carvalho DM, Mackay A, Jones C, Carcaboso AM, Nazarian J, Berens ME, Brenner C, Bindra RS. Nat Commun. 2019 Aug 22;10(1):3790. doi: 10.1038/s41467-019-11732-6. PubMed
NFAT primes the human RORC locus for RORgammat expression in CD4(+) T cells. Yahia-Cherbal H, Rybczynska M, Lovecchio D, Stephen T, Lescale C, Placek K, Larghero J, Rogge L, Bianchi E. Nat Commun. 2019 Oct 16;10(1):4698. doi: 10.1038/s41467-019-12680-x. PubMed
Characterization and mutagenesis of Chinese hamster ovary cells endogenous retroviruses to inactivate viral particle release. Duroy PO, Bosshard S, Schmid-Siegert E, Neuenschwander S, Arib G, Lemercier P, Masternak J, Roesch L, Buron F, Girod PA, Xenarios I, Mermod N. Biotechnol Bioeng. 2020 Feb;117(2):466-485. doi: 10.1002/bit.27200. Epub 2019 Nov 12. PubMed
Tau interactome analyses in CRISPR-Cas9 engineered neuronal cells reveal ATPase-dependent binding of wild-type but not P301L Tau to non-muscle myosins. Wang X, Williams D, Muller I, Lemieux M, Dukart R, Maia IBL, Wang H, Woerman AL, Schmitt-Ulms G. Sci Rep. 2019 Nov 7;9(1):16238. doi: 10.1038/s41598-019-52543-5. PubMed
A substitution mutation in a conserved domain of mammalian acetate-dependent acetyl CoA synthetase 2 results in destabilized protein and impaired HIF-2 signaling. Nagati JS, Xu M, Garcia T, Comerford SA, Hammer RE, Garcia JA. PLoS One. 2019 Nov 14;14(11):e0225105. doi: 10.1371/journal.pone.0225105. eCollection 2019. PubMed
MYCN amplification and ATRX mutations are incompatible in neuroblastoma. Zeineldin M, Federico S, Chen X, Fan Y, Xu B, Stewart E, Zhou X, Jeon J, Griffiths L, Nguyen R, Norrie J, Easton J, Mulder H, Yergeau D, Liu Y, Wu J, Van Ryn C, Naranjo A, Hogarty MD, Kaminski MM, Valentine M, Pruett-Miller SM, Pappo A, Zhang J, Clay MR, Bahrami A, Vogel P, Lee S, Shelat A, Sarthy JF, Meers MP, George RE, Mardis ER, Wilson RK, Henikoff S, Downing JR, Dyer MA. Nat Commun. 2020 Feb 14;11(1):913. doi: 10.1038/s41467-020-14682-6. PubMed
The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Mondal B, Jin H, Kallappagoudar S, Sedkov Y, Martinez T, Sentmanat MF, Poet GJ, Li C, Fan Y, Pruett-Miller SM, Herz HM. Elife. 2020 Apr 16;9. pii: 57519. doi: 10.7554/eLife.57519. PubMed
CRISPR/dCas9-based Scn1a gene activation in inhibitory neurons ameliorates epileptic and behavioral phenotypes of Dravet syndrome model mice. Yamagata T, Raveau M, Kobayashi K, Miyamoto H, Tatsukawa T, Ogiwara I, Itohara S, Hensch TK, Yamakawa K. Neurobiol Dis. 2020 Jul;141:104954. doi: 10.1016/j.nbd.2020.104954. Epub 2020 May 21. PubMed
KRAB-Induced Heterochromatin Effectively Silences PLOD2 Gene Expression in Somatic Cells and is Resilient to TGFbeta1 Activation. Gjaltema RAF, Goubert D, Huisman C, Pilar Garcia Tobilla CD, Koncz M, Jellema PG, Wu D, Brouwer U, Kiss A, Verschure PJ, Bank RA, Rots MG. Int J Mol Sci. 2020 May 21;21(10). pii: ijms21103634. doi: 10.3390/ijms21103634. PubMed
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing. Weisheit I, Kroeger JA, Malik R, Klimmt J, Crusius D, Dannert A, Dichgans M, Paquet D. Cell Rep. 2020 May 26;31(8):107689. doi: 10.1016/j.celrep.2020.107689. PubMed
Telomere Dysfunction Activates p53 and Represses HNF4alpha Expression Leading to Impaired Human Hepatocyte Development and Function. Munroe M, Niero EL, Fok WC, Vessoni AT, Jeong HC, Brenner KA, Batista LFZ. Hepatology. 2020 Jun 9. doi: 10.1002/hep.31414. PubMed
A point mutation in the nuclease domain of MLH3 eliminates repeat expansions in a mouse stem cell model of the Fragile X-related disorders. Hayward BE, Steinbach PJ, Usdin K. Nucleic Acids Res. 2020 Aug 20;48(14):7856-7863. doi: 10.1093/nar/gkaa573. PubMed
Boundary sequences flanking the mouse tyrosinase locus ensure faithful pattern of gene expression. Seruggia D, Fernandez A, Cantero M, Fernandez-Minan A, Gomez-Skarmeta JL, Pelczar P, Montoliu L. Sci Rep. 2020 Sep 23;10(1):15494. doi: 10.1038/s41598-020-72543-0. PubMed
In Vitro Validation of Transgene Expression in Gene-Edited Pigs Using CRISPR Transcriptional Activators. Polkoff KM, Chung J, Simpson SG, Gleason K, Piedrahita JA. CRISPR J. 2020 Oct;3(5):409-418. doi: 10.1089/crispr.2020.0037. PubMed
Cis-acting lnc-eRNA SEELA directly binds histone H4 to promote histone recognition and leukemia progression. Fang K, Huang W, Sun YM, Chen TQ, Zeng ZC, Yang QQ, Pan Q, Han C, Sun LY, Luo XQ, Wang WT, Chen YQ. Genome Biol. 2020 Nov 3;21(1):269. doi: 10.1186/s13059-020-02186-x. PubMed
Tagging Proteins with Fluorescent Reporters Using the CRISPR/Cas9 System and Double-Stranded DNA Donors. Geny S, Pichard S, Brion A, Renaud JB, Jacquemin S, Concordet JP, Poterszman A. Methods Mol Biol. 2021;2247:39-57. doi: 10.1007/978-1-0716-1126-5_3. PubMed
TLR3 controls constitutive IFN-beta antiviral immunity in human fibroblasts and cortical neurons. Gao D, Ciancanelli MJ, Zhang P, Harschnitz O, Bondet V, Hasek M, Chen J, Mu X, Itan Y, Cobat A, Sancho-Shimizu V, Bigio B, Lorenzo L, Ciceri G, McAlpine J, Anguiano E, Jouanguy E, Chaussabel D, Meyts I, Diamond MS, Abel L, Hur S, Smith GA, Notarangelo L, Duffy D, Studer L, Casanova JL, Zhang SY. J Clin Invest. 2021 Jan 4;131(1). pii: 134529. doi: 10.1172/JCI134529. PubMed
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SARM1 is required in human derived sensory neurons for injury-induced and neurotoxic axon degeneration. Chen YH, Sasaki Y, DiAntonio A, Milbrandt J. Exp Neurol. 2021 May;339:113636. doi: 10.1016/j.expneurol.2021.113636. Epub 2021 Feb 4. PubMed
Identification and characterization of a novel mutant isocitrate dehydrogenase 1 inhibitor for glioma treatment. Zhang N, Zheng B, Yao X, Huang X, Du J, Shen Y, Huang Z, Chen J, Lin Q, Lan W, Lin W, Ma W. Biochem Biophys Res Commun. 2021 Apr 30;551:38-45. doi: 10.1016/j.bbrc.2021.02.112. Epub 2021 Mar 11. PubMed
DNA templates with blocked long 3' end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci. Bandyopadhyay S, Douglass J, Kapell S, Khan N, Feitosa-Suntheimer F, Klein JA, Temple J, Brown-Culbertson J, Tavares AH, Saeed M, Lau NC. G3 (Bethesda). 2021 May 14. pii: 6275753. doi: 10.1093/g3journal/jkab169. PubMed
Loss of ATRX confers DNA repair defects and PARP inhibitor sensitivity. Garbarino J, Eckroate J, Sundaram RK, Jensen RB, Bindra RS. Transl Oncol. 2021 Sep;14(9):101147. doi: 10.1016/j.tranon.2021.101147. Epub 2021 Jun 9. PubMed
Folate receptor alpha increases chemotherapy resistance through stabilizing MDM2 in cooperation with PHB2 that is overcome by MORAb-202 in gastric cancer. Sakai H, Kawakami H, Teramura T, Onodera Y, Somers E, Furuuchi K, Uenaka T, Kato R, Nakagawa K. Clin Transl Med. 2021 Jun;11(6):e454. doi: 10.1002/ctm2.454. PubMed
Unraveling Ewing sarcoma tumorigenesis originating from patient-derived Mesenchymal Stem Cells. Sole A, Grossetete S, Heintze M, Babin L, Zaidi S, Revy P, Renouf B, De Cian A, Giovannangeli C, Pierre-Eugene C, Janoueix-Lerosey I, Couronne L, Kaltenbach S, Tomishima M, Jasin M, Grunewald TGP, Delattre O, Surdez D, Brunet E. Cancer Res. 2021 Aug 2. pii: 0008-5472.CAN-20-3837. doi: 10.1158/0008-5472.CAN-20-3837. PubMed
SLFN11 is Widely Expressed in Pediatric Sarcoma and Induces Variable Sensitization to Replicative Stress Caused By DNA-Damaging Agents. Gartrell J, Mellado-Largarde M, Clay MR, Bahrami A, Sahr NA, Sykes A, Blankenship K, Hoffmann L, Xie J, Cho HP, Twarog N, Connelly M, Yan KK, Yu J, Porter SN, Pruett-Miller SM, Neale G, Tinkle CL, Federico SM, Stewart EA, Shelat AA. Mol Cancer Ther. 2021 Nov;20(11):2151-2165. doi: 10.1158/1535-7163.MCT-21-0089. Epub 2021 Aug 19. PubMed
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Associated Plasmids

Functional Validation of the Putative Oncogenic Activity of PLAU. Sarno F, Goubert D, Logie E, Rutten MGS, Koncz M, Deben C, Niemarkt AE, Altucci L, Verschure PJ, Kiss A, Berghe WV, Rots MG. Biomedicines. 2022 Dec 30;11(1):102. doi: 10.3390/biomedicines11010102. PubMed
The mechanosensitive ion channel Piezo1 modulates the migration and immune response of microglia. Zhu T, Guo J, Wu Y, Lei T, Zhu J, Chen H, Kala S, Wong KF, Cheung CP, Huang X, Zhao X, Yang M, Sun L. iScience. 2023 Jan 16;26(2):105993. doi: 10.1016/j.isci.2023.105993. eCollection 2023 Feb 17. PubMed
RTF2 controls replication repriming and ribonucleotide excision at the replisome. Conti BA, Ruiz PD, Broton C, Blobel NJ, Kottemann MC, Sridhar S, Lach FP, Wiley T, Sasi NK, Carroll T, Smogorzewska A. bioRxiv. 2023 Mar 13:2023.03.13.532415. doi: 10.1101/2023.03.13.532415. Preprint. PubMed
Encephalitis and poor neuronal death-mediated control of herpes simplex virus in human inherited RIPK3 deficiency. Liu Z, Garcia Reino EJ, Harschnitz O, Guo H, Chan YH, Khobrekar NV, Hasek ML, Dobbs K, Rinchai D, Materna M, Matuozzo D, Lee D, Bastard P, Chen J, Lee YS, Kim SK, Zhao S, Amin P, Lorenzo L, Seeleuthner Y, Chevalier R, Mazzola L, Gay C, Stephan JL, Milisavljevic B, Boucherit S, Rozenberg F, Perez de Diego R, Dix RD, Marr N, Beziat V, Cobat A, Aubart M, Abel L, Chabrier S, Smith GA, Notarangelo LD, Mocarski ES, Studer L, Casanova JL, Zhang SY. Sci Immunol. 2023 Apr 21;8(82):eade2860. doi: 10.1126/sciimmunol.ade2860. Epub 2023 Apr 21. PubMed
Identification of the EH CRISPR-Cas9 system on a metagenome and its application to genome engineering. Esquerra-Ruvira B, Baquedano I, Ruiz R, Fernandez A, Montoliu L, Mojica FJM. Microb Biotechnol. 2023 Jul;16(7):1505-1523. doi: 10.1111/1751-7915.14266. Epub 2023 Apr 25. PubMed
A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease. Schaaf CR, Polkoff KM, Carter A, Stewart AS, Sheahan B, Freund J, Ginzel J, Snyder JC, Roper J, Piedrahita JA, Gonzalez LM. FASEB J. 2023 Jun;37(6):e22975. doi: 10.1096/fj.202300223R. PubMed
APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels. Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS. CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub 2023 Sep 6. PubMed

Associated Plasmids

Targeted epigenetic silencing of UCHL1 expression suppresses collagen-1 production in human lung epithelial cells. Wu DD, Lau ATY, Xu YM, Reinders-Luinge M, Koncz M, Kiss A, Timens W, Rots MG, Hylkema MN. Epigenetics. 2023 Dec;18(1):2175522. doi: 10.1080/15592294.2023.2175522. Epub 2023 Feb 22. PubMed
Highly Efficient Ex Vivo Correction of COL7A1 through Ribonucleoprotein-Based CRISPR/Cas9 and Homology-Directed Repair to Treat Recessive Dystrophic Epidermolysis Bullosa. Berthault C, Gaucher S, Gouin O, Schmitt A, Chen M, Woodley D, Titeux M, Hovnanian A, Izmiryan A. J Invest Dermatol. 2023 Dec 1:S0022-202X(23)03117-2. doi: 10.1016/j.jid.2023.10.035. PubMed

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