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p6722 MSCV-IP N FlagHA 16E6 I128T
(Plasmid #44154)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44154 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV-IP N-FlagHA
  • Backbone size w/o insert (bp) 8100
  • Total vector size (bp) 8600
  • Modifications to backbone
    Retroviral vector for Flag-HA-tagged HPV16 E6 I128T mutant expression (deficient in E6AP binding). No starting ATG in viral ORF, translation begins from tag only. Puro resistance protein expressed from an IRES.
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HPV16E6
  • Species
    HPV16
  • Insert Size (bp)
    500
  • Mutation
    HPV16 E6 I128T mutant
  • Promoter MSCV LTR
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Paul Lambert - University of Wisconsin, Madison
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p6722 MSCV-IP N FlagHA 16E6 I128T was a gift from Peter Howley (Addgene plasmid # 44154 ; http://n2t.net/addgene:44154 ; RRID:Addgene_44154)
  • For your References section:

    Comprehensive analysis of host cellular interactions with human papillomavirus E6 proteins identifies new E6 binding partners and reflects viral diversity. White EA, Kramer RE, Tan MJ, Hayes SD, Harper JW, Howley PM. J Virol. 2012 Dec;86(24):13174-86. doi: 10.1128/JVI.02172-12. Epub 2012 Sep 26. 10.1128/JVI.02172-12 PubMed 23015706