pcDNA3.1-Alpha-0 Dronpa
(Plasmid #44193)

• Depositing Lab: Narasimhan Gautam
• Publication: Chisari et al J Biol Chem. 2007 Aug 17;282(33):24092-8. Epub 2007 Jun 18.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6493
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein alpha 0
• Species: R. norvegicus (rat)
• Insert Size (bp): 1065
• Mutation: Introduced KpnI and SpeI sites between 92nd and 93rd amino acids of alpha 0
• GenBank ID: NM_017327.1
• Entrez Gene: Gnao1 (a.k.a. Gnao, Hg1g, RATBPGTPC)
• Promoter: CMV
• Tag / Fusion Protein:
  • Dronpa

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: BGH primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dronpa was inserted between amino acid 92 and 93 using the KpnI and SpeI sites which were engineered between these sites.