This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCsy4
(Plasmid #44252)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44252 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Total vector size (bp) 3392
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    csy4 gene
  • Species
    P. aeruginosa
  • Promoter pLtetO-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer gagaaaagaattcaaaAGATCTaaagaggagaaaGGATCTATGGACCACTACCTC
  • 3′ sequencing primer agatccttactcgagttaTCAGAACCAGGGAACGAAACCTCCTTTGC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCsy4 was a gift from Adam Arkin & Stanley Qi (Addgene plasmid # 44252)
  • For your References section:

    RNA processing enables predictable programming of gene expression. Qi L, Haurwitz RE, Shao W, Doudna JA, Arkin AP. Nat Biotechnol. 2012 Oct;30(10):1002-6. doi: 10.1038/nbt.2355. Epub 2012 Sep 16. 10.1038/nbt.2355 PubMed 22983090