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pFIN-XOPS-tdTOM
(Plasmid #44359)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44359 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTY
  • Backbone manufacturer
    LJ Chang
  • Backbone size w/o insert (bp) 8760
  • Total vector size (bp) 11600
  • Modifications to backbone
    Final pFIN backbone: Added cPPT-FLAP sequence upstream of MCS in pTY to create pTYF backbone (Coleman et al 2002 Physiol Genomics 12:221-228). Added 2x250 chicken β-globin HS4 core sequence into 3'LTR of pTYF backbone to create pFIN (Semple-Rowland et al 2007 Mol Vis 13:2001-2011).
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XOPS-tdTOM
  • Alt name
    Xenopus Opsin Promoter
  • Alt name
    tdTomato
  • Species
    X. laevis (frog), Synthetic
  • Insert Size (bp)
    2875
  • Promoter Xenopus Opsin (XOPS) (pos 3125-4501)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    XOPS1.3 promoter (1370 bp)was amplified from XOPS1.3p44/GFP obtained from Dr. Barry Knox.
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFIN-XOPS-tdTOM was a gift from Susan Semple-Rowland (Addgene plasmid # 44359 ; http://n2t.net/addgene:44359 ; RRID:Addgene_44359)
  • For your References section:

    Expression characteristics of dual-promoter lentiviral vectors targeting retinal photoreceptors and Muller cells. Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, Pachigar K, Ludlow R, Khani SC, Smith WC. Mol Vis. 2010 May 27;16:916-34. PubMed 20517486