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pHEX2
(Plasmid #44559)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44559 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pART27
  • Backbone manufacturer
    Gleave AP (1992) Plant Mol Biol 20:1203-1207
  • Backbone size (bp) 11608
  • Modifications to backbone
    Gateway conversion of pART27
  • Vector type
    Plant Transformation Vector
  • Promoter CaMV35S
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses

Depositor Comments

pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13

pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHEX2 was a gift from Andrew Gleave & Roger Hellens (Addgene plasmid # 44559)
  • For your References section:

    Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Hellens RP, Allan AC, Friel EN, Bolitho K, Grafton K, Templeton MD, Karunairetnam S, Gleave AP, Laing WA. Plant Methods. 2005 Dec 18;1:13. 10.1186/1746-4811-1-13 PubMed 16359558