pIC26
(Plasmid #44572)

• Purpose: (Empty Backbone)
• Depositing Labs: Iain Cheeseman, Arshad Desai
• Publication: Cheeseman et al Sci STKE. 2005 Jan 11;2005(266):pl1.

Backbone

• Vector backbone: bluescript
• Modifications to backbone: N terminal GFP, tev and s peptide
• Vector type: Worm Expression, Retroviral
• Promoter: pie-1
• Selectable markers: unc119
• Tags / Fusion Proteins:
  • GFP (N terminal on insert)
  • tev (N terminal on insert)
  • s peptide (N terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: GFP-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tandem Affinity Purification plasmid - pIC26 allows a target protein to be fused at its N-terminus to the GFP-Tev-S peptide tag using the SpeI restriction site. The plasmid contains pie-1 regulatory sequences to express the tagged protein in the germline and embryo and can be integrated by ballistic bombardment of the strain DP38 using unc-119 as a transformation marker. See Cheeseman et al., Genes Dev. 2004 Sep 15;18(18):2255-68 (https://www.ncbi.nlm.nih.gov/pubmed/15371340) for more information.