Hsp70-mplum
(Plasmid #45016)

• Depositing Lab: Anna Planas
• Publication: de la Rosa et al Eur J Nucl Med Mol Imaging. 2013 Feb;40(3):426-38. doi: 10.1007/s00259-012-2277-7. Epub 2012 Nov 8.

Backbone

• Vector backbone: pDEST-R4-R3
• Backbone manufacturer: Life Technologies/Invitrogen
• Total vector size (bp): 4511
• Modifications to backbone: The attB1 site resulting from MultiSite Gateway recombination was lost owing to further modification in order to insert the beta-globin intron between the Hspa1b promoter and the mPlum coding sequence.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mPlum
• Species: Discosoma sp.
• Insert Size (bp): 681
• GenBank ID: AY786537.1
• Promoter: mouse Hspa1b

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward (-20)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original mPlum cDNA for constructing our plasmid was initially provided by Dr Roger Y.Tsien (UCSD), and use was subject to their MTA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Wang L, Jackson WC, Steinbach PA, Tsien RY. Evolution of new nonantibody
proteins via iterative somatic hypermutation. Proc Natl Acad Sci U S A. 2004 Nov
30;101(48):16745-9.