pCI-neo:alpha4-mcherry
(Plasmid #45073)

• Depositing Lab: Henry Lester
• Publication: Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 8069
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha4-mcherry
• Alt name: nAChR alpha4-mcherry
• Species: M. musculus (mouse)
• Insert Size (bp): 2597
• Entrez Gene: Chrna4 (a.k.a. Acra-4, Acra4, EBN1, ENFL1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: J.Stizel (University of Colorado, Boulder, CO)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Principal Investigator Henry A. Lester

α4 cloned into EcoRI sites of pCI-neo