pR208IIM1
(Plasmid #45352)

• Depositing Lab: Alexander Varshavsky
• Publication: Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-28.

Backbone

• Vector backbone: pBR208
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: UBR1 mutant
• Alt name: empty ADH 2 micron LEU UBR1 type II mutant IIM1
• Mutation: P406S, DE317NK
• Promoter: ADH

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: BglII (unknown if destroyed)
• 5′ sequencing primer: yADH1-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid overexpresses a mutant Ubr1pha that is able to target type I but not type II N-end rule substrates for degradation.

Mutation is a single base change resulting in a P to S change in CLIPNSS -> CLISNSS