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IF-GFP-ATRX
(Plasmid #45444)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45444 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 12000
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    As ATRX is prone to insertion by IS10, you should sequence exon 8 and the entire sequence on occasion but especially after culturing in E. coli. A good primer for sequencing is GCCAACATGAACTCCAGTTATG.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATRX
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    7400
  • Mutation
    isoform 2, missing codon E124
  • Entrez Gene
    ATRX (a.k.a. JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX)
  • Promoter PCMV
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP N sequencing primer
  • 3′ sequencing primer EGFP C sequencing primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    It was made from a combination of ATRX sequence cloned by David Picketts and a short sequence engineered by Genscript.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

The EcoRI restriction pattern is different between the plasmid described in the paper and the plasmid available from Addgene. This difference does not effect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IF-GFP-ATRX was a gift from Michael Dyer (Addgene plasmid # 45444 ; http://n2t.net/addgene:45444 ; RRID:Addgene_45444)
  • For your References section:

    The ATRX cDNA is prone to bacterial IS10 element insertions that alter its structure. Valle-Garcia D, Griffiths LM, Dyer MA, Bernstein E, Recillas-Targa F. Springerplus. 2014 May 2;3:222. doi: 10.1186/2193-1801-3-222. eCollection 2014. 10.1186/2193-1801-3-222 PubMed 24834375