PurposeATRX expression vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45444||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepEGFP C2
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 12000
Vector typeMammalian Expression, Bacterial Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth instructionsAs ATRX is prone to insertion by IS10, you should sequence exon 8 and the entire sequence on occasion but especially after culturing in E. coli. A good primer for sequencing is GCCAACATGAACTCCAGTTATG.
Copy numberHigh Copy
SpeciesH. sapiens (human), M. musculus (mouse)
Insert Size (bp)7400
Mutationisoform 2, missing codon E124
Entrez GeneATRX (a.k.a. JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX)
- Promoter PCMV
/ Fusion Proteins
- GFP (N terminal on backbone)
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer EGFP N sequencing primer
- 3′ sequencing primer EGFP C sequencing primer (Common Sequencing Primers)
The EcoRI restriction pattern is different between the plasmid described in the paper and the plasmid available from Addgene. This difference does not effect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:IF-GFP-ATRX was a gift from Michael Dyer (Addgene plasmid # 45444 ; http://n2t.net/addgene:45444 ; RRID:Addgene_45444)
For your References section:The ATRX cDNA is prone to bacterial IS10 element insertions that alter its structure. Valle-Garcia D, Griffiths LM, Dyer MA, Bernstein E, Recillas-Targa F. Springerplus. 2014 May 2;3:222. doi: 10.1186/2193-1801-3-222. eCollection 2014. 10.1186/2193-1801-3-222 PubMed 24834375