pLV-tetO YY1
(Plasmid #45498)

• Depositing Lab: Sean Wu
• Publication: Gregoire et al Circ Res. 2013 Mar 15;112(6):900-10. doi: 10.1161/CIRCRESAHA.113.259259. Epub 2013 Jan 10.

Backbone

• Vector backbone: pLV-tetO (PMID: 18371448)
• Backbone manufacturer: Konrad Hochedlinger lab (MGH, Boston, MA)
• Modifications to backbone: Backbone is modified from FUGW (Addgene plasmid 14883) by replacing the hUbC_promoter-EGFP-containing PacI-EcoRI fragment with tetO-CMV promoter-containing XhoI-EcoRI fragment from pTet-Splice
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YY1
• Alt name: YY1 transcription factor
• Alt name: Yin Yang 1
• Alt name: NF-E1
• Species: H. sapiens (human)
• GenBank ID:
• Entrez Gene: YY1 (a.k.a. DELTA, GADEVS, INO80S, NF-E1, UCRBP, YIN-YANG-1)
• Promoter: TetO-CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: YY1 cDNA from Dr. Edward Seto, Moffitt Cancer Center, Florida. pLV-tetO plasmid backbone from Konrad Hochedlinger, MGH, Boston, MA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry