|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45567||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5729
Modifications to backboneNheI, XhoI, and SacI cloning sites and an encephalomyocarditis virus IRES inserted upstream of EGFP. Puromycin resistance protein (puromycin N-acetyl transferase) fused downstream of EGFP
Vector typeMammalian Expression
- Promoter Phcmv (strong human cytomegalovirus immediate early promoter)
Selectable markersPuromycin ; EGFP
/ Fusion Protein
- IRES-EGFP-Puro (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG) (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
Plasmid pIRES-EGFP-puro was constructed containing the Phcmv promoter followed by unique restriction sites (NheI, XhoI, and SacI) for insertion of genes. Downstream, an IRES from the encephalomyocarditis virus directs the translation of EGFP-puro from the same mRNA.
Addgene's sequencing results found differences compared to the depositing lab's sequence in the 3' region of the puro resistance marker. The resulting protein sequence matches NCBI WP_055528321.1 and should be functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIRES-EGFP-puro was a gift from Michael McVoy (Addgene plasmid # 45567 ; http://n2t.net/addgene:45567 ; RRID:Addgene_45567)