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pD40-His/V5-c-MycS62A
(Plasmid #45599)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45599 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA DEST40
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7143
  • Total vector size (bp) 6841
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Myc S62A
  • Alt name
    c-Myc
  • Alt name
    Myc2
  • Alt name
    myelocytomatosis oncogene
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • Mutation
    S62A
  • Entrez Gene
    Myc (a.k.a. AU016757, Myc2, Niard, Nird, bHLHe39)
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Murine c-myc cDNA amplified from plasmid CMV-Myc from Stephen R. Hann (Vanderbuilt University) (PMID: 7958908) (Murine myc cDNA originally obtained from Michael Cole, Princeton, NJ)
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

Alternate plasmid names: pD40-His-c-MycS62A; pcDNA-DEST40 Myc S62A

Plasmid construction information:
Point mutations were introduced into the murine c-Myc sequence in the CMV–Myc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) using the GeneEditor kit (Promega) per manufacturer's instructions using oligonucletide
MycS62A, 5′-CACCCCGCCCCTGGCCCCGAGC-3′.

The His6-tagged c-MycS62A plasmid was then created by PCR amplification with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-MycS62A.

For more plasmid information (map and sequence), see the wildtype version, pD40-His/V5-c-Myc (Addgene plasmid 45597) For additional usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pD40-His/V5-c-MycS62A was a gift from Rosalie Sears (Addgene plasmid # 45599 ; http://n2t.net/addgene:45599 ; RRID:Addgene_45599)
  • For your References section:

    A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Yeh E, Cunningham M, Arnold H, Chasse D, Monteith T, Ivaldi G, Hahn WC, Stukenberg PT, Shenolikar S, Uchida T, Counter CM, Nevins JR, Means AR, Sears R. Nat Cell Biol. 2004 Apr;6(4):308-18. Epub 2004 Mar 14. 10.1038/ncb1110 PubMed 15048125