GRK2 S670A
(Plasmid #45755)

• Depositing Lab: Robert Lefkowitz
• Publication: Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 4754
• Total vector size (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GRK2 S670A
• Alt name: GRK2
• Alt name: beta adrenergic receptor kinase 1
• Alt name: ADRBK1
• Species: B. taurus (bovine)
• Insert Size (bp): 2300
• Mutation: S670A
• Entrez Gene: GRK2 (a.k.a. ADRBK1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The additional V661G mutation in the Addgene QC sequence does not affect plasmid function.